FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\uspQ288A
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General Information
Symbol
Dmel\uspQ288A
Species
D. melanogaster
Name
FlyBase ID
FBal0291035
Feature type
allele
Associated gene
Dmel\usp
Associated Insertion(s)
Carried in Construct
P{usp.Q288A}
Key Links
Transgenic product class
missense_variant
(Jones et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Jones et al., 2013)
Mutagen
in vitro construct
(Jones et al., 2013)
Progenitor genotype
Carried in construct
P{usp.Q288A}
Cytology
Description

Genomic fragment containing usp with a mutation that disrupts an amino acid (Q288A) that normally extends a side chain into the ligand binding pocket, resulting in a usp protein with reduced affinity for the methyl farnesoate ligand.

(Jones et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
usp
Encoded product / tool
usp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      uspQ288A usp2 animals progress normally through to apolysis of the the epidermis away from the cuticle (albeit with a briefly longer 3rd instar feeding stage compared to normal larvae). Subsequently, uspQ288A usp2 animals do not form a normal puparium - it is dorso-ventrally flattened, the partially everted anterior spiracles point directly forward, the cuticle is only faintly sclerotized, the 'puparial' cuticle is transparent rather than brown, and head eversion is incomplete. Most animals stop development during the early pupal stage and eventually die.

      (Jones et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      uspQ288A partially rescues usp2

      (Jones et al., 2013)
      Comments

      uspQ288A rescues usp2 first instar larval lethality to the prepupal stage: larvae progress normally through to apolysis of the the epidermis away from the cuticle (albeit with a briefly longer 3rd instar feeding stage compared to normal larvae). Subsequently, uspQ288A usp2 animals do not form a normal puparium and most animals stop development during the early pupal stage and eventually die.

      (Jones et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      uspQ288A
      Name Synonyms
      Secondary FlyBase IDs
        References (1)