FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Mi-2Δ932-1158.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Mi-2Δ932-1158.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0291047
Feature type
allele
Associated gene
Dmel\Mi-2
Associated Insertion(s)
Carried in Construct
P{UAS-Mi-2.Δ932-1158.FLAG}
Key Links
Transgenic product class
inframe_deletion
(Fasulo et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Fasulo et al., 2012)
Mutagen
in vitro construct
(Fasulo et al., 2012)
Progenitor genotype
Carried in construct
P{UAS-Mi-2.Δ932-1158.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of Mi-2 which lacks amino acid residues 932-1158 (this removes the bipartite ATP-binding site) and which is tagged at the C-terminal end with Tag:FLAG.

(Fasulo et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mi-2
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The majority of animals expressing Mi-2Δ932-1158.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 complete larval development at 25[o]C, but only 5% survive to adulthood. At 29[o]C no animals survive beyond the first or second larval instar.

      The lethality caused by expression of Mi-2Δ932-1158.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 is moderately enhanced by Mi-2f08103/+ and is strongly enhanced by Mi-24/+.

      Polytene chromosomes in larvae expressing Mi-2Δ932-1158.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32 and shifted from 25 to 29[o]C at the end of the first larval instar are thinner than normal and have a disrupted banding pattern (both in fixed squash preparations and in live analysis).

      Animals carrying Mi-2Δ932-1158.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4da.G32 and Scer\GAL80ts.αTub84B and shifted from 18 to 29[o]C during the third larval instar (which inactivates the temperature sensitive Scer\GAL80ts.αTub84B allele, allowing expression of Mi-2Δ932-1158.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4da.G32) show a significant decrease in polytene chromosome volume and a modest increase in polytene chromosome compaction.

      (Fasulo et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mi-2Δ932-1158.Scer\UAS.T:Zzzz\FLAG
      Mi-2Δ932-1158.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)