FlyBase Allele Report: Hsap\DNAJB1[UAS.cKa]

General Information

Symbol

Hsap\DNAJB1^UAS.cKa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Kuo

FlyBase ID

FBal0291069

Feature type

allele

Associated gene

Hsap\DNAJB1

Associated Insertion(s)

Carried in Construct

P{UAS-DNAJB1.K}

Also Known As

UAS-dnaJB1

Key Links

Transgenic product class

wild_type

(Kuo et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kuo et al., 2013)

Mutagen

in vitro construct

(Kuo et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-DNAJB1.K}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\DNAJB1.

(Kuo et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\DNAJB1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEC with Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

model of Huntington's disease

CEC with Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}, Hsap\HSPA4L^UAS.cKa

(Kuo et al., 2013)

ameliorates Huntington's disease

modeled by Hsap\HTT^GMR.Q120, Hsap\HSPA4L^UAS.cKa

(Kuo et al., 2013)

modeled by Hsap\HTT^{N548.Q128.UAS}

(Prakash et al., 2022, Martin et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\DNAJB1^UAS.cKa, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, suppressible | partially by Hsap\HSPA4L^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

Suppressor of

Statement

Reference

Hsap\DNAJB1^UAS.cKa, Scer\GAL4^{repo.Switch.2301} is a suppressor | partially of short lived | RU486 conditional phenotype of Hsap\HTT^{N548.Q128.UAS}, Scer\GAL4^{repo.Switch.2301}

(Martin et al., 2021)

Hsap\DNAJB1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\DNAJB1^UAS.cKa, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, suppressible | partially by Hsap\HSPA4L^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

Suppressor of

Statement

Reference

Hsap\DNAJB1^UAS.cKa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Hsap\HSPA4L^UAS.cKa, Scer\GAL4^GMR.PF, Hsap\DNAJB1^UAS.cKa is a suppressor of eye phenotype of Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

Hsap\HSPA4L^UAS.cKa, Scer\GAL4^GMR.PF, Hsap\DNAJB1^UAS.cKa is a suppressor of rhabdomere phenotype of Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/Hsap\DNAJB1^UAS.cKa is a non-suppressor of eye phenotype of Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

Scer\GAL4^GMR.PF/Hsap\DNAJB1^UAS.cKa is a non-suppressor of rhabdomere phenotype of Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\DNAJB1^Scer\UAS.cKa partially suppresses the loss of eye pigmentation seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsap\DNAJB1^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF does not suppress the photoreceptor loss phenotype seen upon expression of Hsap\HTT^GMR.Q120. Complete loss of rhabdomeres and severe degeneration of photoreceptor cell is detected in the retinae of 7 day old flies.

Expression of Hsap\HSPA4L^Scer\UAS.cKa further suppresses the loss of eye pigmentation and external degeneration seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and Hsap\DNAJB1^Scer\UAS.cKa are expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of Hsap\DNAJB1^Scer\UAS.cKa and Hsap\HSPA4L^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF partially suppresses the photoreceptor loss phenotype seen upon expression of Hsap\HTT^GMR.Q120. Many normal photoreceptor cells are seen in the retinae of 7 day old flies.

(Kuo et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

53730

w^*; P{UAS-DNAJB1.K}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\DNAJB1^Scer\UAS.cKa

Hsap\DNAJB1^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kuo

Secondary FlyBase IDs

References (3)

Report Sections

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