FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Syt1C2A*-C2B*.UAS
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General Information
Symbol
Dmel\Syt1C2A*-C2B*.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0291475
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
P{UAS-Syt1.C2A*-C2B*}
Key Links
Transgenic product class
missense_variant
(Lee et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Lee et al., 2013)
Mutagen
in vitro construct
(Lee et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-Syt1.C2A*-C2B*}
Cytology
Description

UAS regulates expression of Syt1 containing D282N and D284N mutations in the C2A domain and D416N and D418N mutations in the C2B domain, thus preventing Ca[2+] binding both domains.

(Lee et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Syt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4elav-C155-mediated expression of Syt1C2A*-C2B*.Scer\UAS in a Syt1N13/Syt1AD4 background results in spontaneous neurotransmitter release frequency (measured as mEJPs) at third instar NMJs far beyond the levels observed in controls.

      Scer\GAL4elav-C155-mediated expression of Syt1C2A*-C2B*.Scer\UAS results in a dramatic increase in nerve-evoked excitatory junction potential (eEJP) amplitude without affecting asynchronous release or spontaneous fusion (as measured by mEJPs) at third instar NMJs at low (0.2mM) external Ca[2+], compared to controls.

      There are no discernible synaptic abnormalities associated with over-expression of Syt1C2A*-C2B*.Scer\UAS, including no significant increase in the number of synaptic vesicles near active zones, compared to over-expression of Syt1C2A-C2B.Scer\UAS. There is also no significant change in the number of release sites between these two genotypes.

      Scer\GAL4elav-C155-mediated expression of Syt1C2A*-C2B*.Scer\UAS increases vesicle release probability and lowers the Ca[2+] concentration required to trigger fusion, compared to expression of Syt1C2A-C2B.Scer\UAS.

      (Lee et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4elav-C155/Syt1C2A*-C2B*.UAS partially rescues Syt1AD4/Syt1N13

      (Lee et al., 2013)
      Fails to rescue

      Scer\GAL4elav-C155/Syt1C2A*-C2B*.UAS fails to rescue Syt1AD4/Syt1N13

      (Lee et al., 2013)
      Comments

      Scer\GAL4elav-C155-mediated expression of Syt1C2A*-C2B*.Scer\UAS fails to restore synchronous neurotransmitter release (measured as eEJPs) at third instar NMJs in Syt1N13/Syt1AD4 animals at either low (0.2mM) or high (1.0mM) external Ca[2+].

      Scer\GAL4elav-C155-mediated expression of Syt1C2A*-C2B*.Scer\UAS fails to restore spontaneous neurotransmitter release frequency (measured as mEJPs) to control levels at third instar NMJs in Syt1N13/Syt1AD4 animals at low (0.2mM) external Ca[2+]. Rather, mEJP frequency is dramatically increased.

      Scer\GAL4elav-C155-mediated expression of Syt1C2A*-C2B*.Scer\UAS partially rescues the reduction in docked synaptic vesicles and vesicle density at type 1b boutons in Syt1N13/Syt1AD4 third instar larvae.

      (Lee et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Syt1C2A*-C2B*.Scer\UAS
      Syt1C2A*-C2B*.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)