FlyBase Allele Report: Dmel\wfs1[HMC03559]

General Information

Symbol

Dmel\wfs1^HMC03559

Species

D. melanogaster

Name

FlyBase ID

FBal0292694

Feature type

allele

Associated gene

Dmel\wfs1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMC03559}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMC03559}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMC03559

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Wolfram syndrome 1

CEC

(Sakakibara et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Wolfram syndrome 1

is exacerbated by Eaat1^SM2

(Sakakibara et al., 2018)

exacerbates Alzheimer's disease

modeled by Hsap\MAPT^UAS.cSa

(Sakakibara et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "Wolfram syndrome 1" is associated with human ortholog WFS1.

(Sakakibara et al., 2018)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult central brain | progressive, with Scer\GAL4^elav-C155

(Sakakibara et al., 2018)

adult central brain | progressive, with Scer\GAL4^elav-C155, Scer\GAL4^repo

(Sakakibara et al., 2018)

adult optic lobe | progressive, with Scer\GAL4^elav-C155

(Sakakibara et al., 2018)

adult optic lobe | progressive, with Scer\GAL4^elav-C155, Scer\GAL4^repo

(Sakakibara et al., 2018)

Detailed Description

Statement

Reference

Adults expressing wfs1^HMC03559 under the control of Scer\GAL4^elav-C155 alone or under the combined control of Scer\GAL4^elav-C155 and Scer\GAL4^repo (but not when under the control of Scer\GAL4^repo alone) show age-dependent climbing defects and brain vacuolization (optic lobe and central neuropil), as compared to controls; these phenotypes are more severe under the combined control of Scer\GAL4^elav-C155 and Scer\GAL4^repo.

Adults expressing wfs1^HMC03559 under the control of Scer\GAL4^elav-C155 alone or under the combined control of Scer\GAL4^elav-C155 and Scer\GAL4^repo show a severely impaired righting reflex, as compared to controls.

Adults expressing wfs1^HMC03559 under the control of Scer\GAL4^elav-C155 alone present mushroom body neurons without mitochondria distribution defects within the cell body.

Adults expressing wfs1^HMC03559 under the control of Scer\GAL4^GMR.PF do not show lamina defects.

(Sakakibara et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, wfs1^HMC03559 has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Eaat1[+]/Eaat1^SM2

(Sakakibara et al., 2018)

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, wfs1^HMC03559 has abnormal locomotor behavior | adult stage | progressive phenotype, non-enhanceable by Opa1[+]/Opa1^s3475

(Sakakibara et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, wfs1^HMC03559 has abnormal locomotor behavior | adult stage | progressive phenotype, non-suppressible by Opa1[+]/Opa1^s3475

(Sakakibara et al., 2018)

Enhancer of

Statement

Reference

wfs1^HMC03559, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\MAPT^UAS.cSa, Scer\GAL4^GMR.PF

(Sakakibara et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

wfs1^HMC03559, Scer\GAL4^GMR.PF is an enhancer of lamina | adult stage | progressive phenotype of Hsap\MAPT^UAS.cSa, Scer\GAL4^GMR.PF

(Sakakibara et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

53330

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03559}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wfs1^HMC03559

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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