FlyBase Allele Report: Dmel\Opa1[s3475]

(Ponte et al., 2020, Terriente-Felix et al., 2020, Duboff et al., 2012, Yarosh et al., 2008, McQuibban et al., 2006, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Heterozygous mutant flies have lower levels of opa1-like protein than controls.

(Yarosh et al., 2008)

Insertion components

P{lacW}Opa1^s3475

Product class / Tool use(s)

Encoded product / tool

Tagged with

nuclear protein localization tag

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of optic atrophy

CEA

(Yarosh et al., 2008)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Poole et al., 2008)

modeled by park²⁵, park^Z472

(Poole et al., 2008)

modeled by park²⁵

(Poole et al., 2008)

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Duboff et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | dominant

(Smith et al., 2019)

lethal

(Yarosh et al., 2008)

lethal | recessive

(Spradling et al., 1999)

lethal - all die before end of first instar larval stage

(McQuibban et al., 2006)

partially lethal - majority die

some die during first instar larval stage

(McQuibban et al., 2006)

Phenotype Manifest In

axon | adult stage

(Smith et al., 2019)

eye | somatic clone

(Yarosh et al., 2008)

eye | somatic clone - Minute background

(Yarosh et al., 2008)

mitochondrion | adult stage

(Smith et al., 2019)

mitochondrion | germline clone

(McQuibban et al., 2006)

Nebenkern | germline clone

(McQuibban et al., 2006)

Detailed Description

Statement

Reference

Opa1^s3475/+ heterozygous mutants have significantly reduced mitochondrial length and significantly increased mitochondrial number in axons of adult neurons, compared to controls.

(Smith et al., 2019)

Heterozygotes do not show gross eye phenotypes.

Small homozygous eye clones have a slightly rough appearance at low penetrance.

Large homozygous eye clones (generated in a Minute background) exhibit a robust rough and glossy appearance, together with tissue necrosis of variable onset and penetrance.

(Yarosh et al., 2008)

opa1-like^s3475 mitotic clones in the male germline (i.e. mutant spermatids) exhibit irregularly-shaped nebenkerns, composed of many individual mitochondria that have coalesced beside the nucleus, but have failed to fuse.

(McQuibban et al., 2006)

visible: wings held out at 45 degree angle

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Opa1^s3475 has abnormal neuroanatomy | dominant | adult stage phenotype, suppressible | partially | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Enhancer of

Statement

Reference

Opa1[+]/Opa1^s3475 is an enhancer of visible phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Poole et al., 2008)

NOT Enhancer of

Statement

Reference

Opa1[+]/Opa1^s3475 is a non-enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^elav-C155, wfs1^HMC03559

(Sakakibara et al., 2018)

Suppressor of

Statement

Reference

Opa1[+]/Opa1^s3475 is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^da.G32, fwd^GL00145

(Terriente-Felix et al., 2020)

Opa1[+]/Opa1^s3475 is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Opa1^s3475 is a suppressor of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Opa1^s3475 is a suppressor of abnormal cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Opa1^s3475 is a suppressor | partially of abnormal flight phenotype of Pink1^B9

(Vilain et al., 2012)

Opa1[+]/Opa1^s3475 is a suppressor | partially of abnormal flight phenotype of park^Z472/park²⁵

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of visible phenotype of Pink1^B9

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of abnormal flight phenotype of Pink1^B9

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of abnormal locomotor behavior phenotype of Pink1^B9

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of abnormal locomotor behavior phenotype of park²⁵

(Poole et al., 2008)

NOT Suppressor of

Statement

Reference

Opa1[+]/Opa1^s3475 is a non-suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^elav-C155, wfs1^HMC03559

(Sakakibara et al., 2018)

Opa1^s3475 is a non-suppressor of male sterile phenotype of Pink1^B9

(Vilain et al., 2012)

Opa1[+]/Opa1^s3475 is a non-suppressor of abnormal flight phenotype of park²⁵

(Poole et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Opa1^s3475 has mitochondrion | adult stage phenotype, suppressible | partially | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Opa1^s3475 has axon | adult stage phenotype, suppressible | partially | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Enhancer of

Statement

Reference

Opa1[+]/Opa1^s3475 is an enhancer of eye phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Poole et al., 2008)

Suppressor of

Statement

Reference

Opa1[+]/Opa1^s3475 is a suppressor of mitochondrion | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Opa1[+]/Opa1^s3475 is a suppressor of axon | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Opa1^s3475 is a suppressor of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Opa1^s3475 is a suppressor of mitochondrion phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Opa1[+]/Opa1^s3475 is a suppressor | partially of adult thorax phenotype of park^Z472/park²⁵

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of mitochondrion phenotype of Pink1^B9

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor | partially of adult thorax phenotype of park²⁵

(Poole et al., 2008)

Opa1[+]/Opa1^s3475 is a suppressor of mitochondrion phenotype of park²⁵

(Poole et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Opa1^s3475/+ fully suppresses the mitochondrial morphology phenotype, and partially suppresses the thoracic indentation and impaired climbing phenotypes (but not impaired flight) of park²⁵/park²⁵ mutants, and partially suppresses the thoracic indentation and impaired flight phenotypes of park²⁵/park^Z472 mutants.

Opa1^s3475/+ partially suppresses the thoracic indentation, impaired flight, impaired climbing, and mitochondrial morphology phenotypes of Pink1^B9 hemizygous mutants.

The rough eye phenotype of flies expressing Pink1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is enhanced in the presence of Opa1^s3475/+ mutation.

(Poole et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of opa1-like^s3475 significantly suppresses the increase in mitochondria length seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU. The increased neurotoxicity is also significantly rescued.

(Duboff et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12188

w¹¹¹⁸; P{lacW}Opa1^s3475/CyO

Kyoto

111438

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Opa1^s3475 /CyO y⁺

Notes on Origin

Discoverer

M. Scott.

Comments

Complements: l(2)00572⁰⁰⁵⁷². Complements: cg⁰⁷⁶⁵⁹. Complements: Hsc70-5^k04907. Complements: l(2)k05416^k05416. Complements: Hsc70-5^k06618. Complements: SelD^k11320. Complements: SelD^k12303.

(BDGP Project Members, 1994-1999)

Excision of the P{lacW} element completely reverses the eye phenotype in somatic clones.

(Yarosh et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

OPA1-like^s3475

(Duboff et al., 2012)

Opa1 ^s3475

(Smith et al., 2019)

Opa1^S3475

(Sakakibara et al., 2018)

Opa1^s3475

(Chen et al., 2020, Ponte et al., 2020, Terriente-Felix et al., 2020)

dOpa1^ex2

(Yarosh et al., 2008)

l(2)s3475

(Spradling et al., 1999)

l(2)s3475^s3475

opa1-like³⁴⁷⁵

(Vilain et al., 2012)

opa1-like^s3475

opa1^S3

(Vilain et al., 2012, Poole et al., 2008)

opa1^l(2)S3475

(Poole et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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