FlyBase Allele Report: Dmel\cmet[HMJ21427]

General Information

Symbol

Dmel\cmet^HMJ21427

Species

D. melanogaster

Name

FlyBase ID

FBal0293156

Feature type

allele

Associated gene

Dmel\cmet

Associated Insertion(s)

Carried in Construct

P{TRiP.HMJ21427}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMJ21427}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMJ21427

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cUa, Scer\GAL4^ap.PU, cmet^HMJ21427 has abnormal endomitotic cell cycle phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Scer\GAL4^ap.PU, cmet^HMJ21427 has neoplasia phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Scer\GAL4^en.PU, cmet^HMJ21427 has abnormal endomitotic cell cycle phenotype

(Clemente-Ruiz et al., 2014)

BacA\p35^UAS.cUa, Scer\GAL4^en.PU, cmet^HMJ21427 has neoplasia phenotype

(Clemente-Ruiz et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of cmet^HMJ21427 and BacA\p35^Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4^ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35^Scer\UAS.cUa-expressing control larvae and ultimately die.

Expression of cmet^HMJ21427 and BacA\p35^Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4^en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35^Scer\UAS.cUa-expressing control larvae and ultimately die.

(Clemente-Ruiz et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

54004

y¹ v¹; P{TRiP.HMJ21427}attP40

NIG-Fly

HMJ21427