FlyBase Allele Report: Dmel\α-Cat[UASp.Tag:HA]

General Information

Symbol

Dmel\α-Cat^UASp.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0294017

Feature type

allele

Associated gene

Dmel\α-Cat

Associated Insertion(s)

Carried in Construct

P{UASp-α-Cat.HA}

Also Known As

UAS-α-Cat, αCat::HA

Key Links

Transgenic product class

cDNA

(Sarpal et al., 2012)

wild_type

(Sarpal et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sarpal et al., 2012)

wild_type

(Sarpal et al., 2012)

Mutagen

in vitro construct

(Sarpal et al., 2012)

Progenitor genotype

Carried in construct

P{UASp-α-Cat.HA}

Cytology

Description

UASp regulatory sequences drive expression of Tag:HA tagged α-Cat cDNA.

(Sarpal et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

α-Cat

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

α-Cat^UASp.Tag:HA, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 is a non-suppressor of lethal - all die during embryonic stage phenotype of α-Cat¹

(Sarpal et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

α-Cat^UASp.Tag:HA/Scer\GAL4^unspecified rescues α-Cat¹

(Escobar et al., 2015)

α-Cat^UASp.Tag:HA, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 rescues α-Cat¹

(Desai et al., 2013)

α-Cat^UASp.Tag:HA, Scer\GAL4^Act.PU, Scer\GAL4^da.G32 rescues α-Cat¹

(Sarpal et al., 2012)

Comments

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^unspecified rescues the embryonic head morphogenesis and lethality of α-Cat¹/α-Cat¹ mutants, and rescues the follicular epithelium integrity defects of α-Cat¹/α-Cat¹ follicle cell clones.

(Escobar et al., 2015)

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the follicular epithelium cell phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also rescued.

Ubiquitous expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

(Desai et al., 2013)

Expression of α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU rescues the embryonic lethality seen in α-Cat¹ mutants to fertile adults.

Expressing α-Cat^{Scer\UAS.P\T.T:Ivir\HA1} allows α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. The follicle epithelium and border cell migration defects are also rescued.

(Sarpal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65590

w¹¹¹⁸; P{UASp-α-Cat.HA}attP2

67414

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-α-Cat.HA}attP2, α-Cat¹/TM6B, Tb¹

Notes on Origin

Discoverer