FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab7T22N.UAS.Venus
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General Information
Symbol
Dmel\Rab7T22N.UAS.Venus
Species
D. melanogaster
Name
FlyBase ID
FBal0294207
Feature type
allele
Associated gene
Dmel\Rab7
Associated Insertion(s)
Carried in Construct
PBac{UAS-Rab7.T22N.Venus}
Also Known As
UAS-Venus-Rab7T22N
Key Links
Genomic Maps

Transgenic product class
dominant_negative_variant
(Cherry et al., 2013)
missense_variant
(Cherry et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Cherry et al., 2013)
missense_variant
(Cherry et al., 2013)
Mutagen
in vitro construct
(Cherry et al., 2013)
Progenitor genotype
Carried in construct
PBac{UAS-Rab7.T22N.Venus}
Cytology
Description

UASt regulatory sequences drive expression of a dominant negative form of Rab7 in which the threonine at amino acid 22 has been replaced by asparagine. The construct is tagged at the N-terminal end with Venus.

(Cherry et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rab7
Tagged with
Venus
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:23,994,066..23,994,066 [+]
Nucleotide change:

C23994066A

Amino acid change:

T22N | Rab7-PA; T22N | Rab7-PB; T22N | Rab7-PC

Reported amino acid change:

T22N

Comment:

Carried in construct; Inserted at PBac{y+-attP-3B}VK00002 landing site on 2L. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Cherry et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  amyotrophic lateral sclerosis type 10
      modeled by Hsap\TARDBPUAS.cUa
      (Liu et al., 2017)
      modeled by Hsap\TARDBPG298S.UAS
      (Liu et al., 2017)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing two copies of Rab7T22N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Rab7.T:Disc\RFP are viable.

      Flies expressing one copy of Rab7T22N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of one copy of Scer\GAL4Rab7.T:Disc\RFP (in a Rab7GAL4.T:Disc\RFP/+ mutant background) are viable and do not display any obvious behavioural defects or altered lifespan. Electroretinogram recordings from the larval neuromuscular junction are indistinguishable from wild type. The frequency and amplitudes of spontaneous single vesicle release events are normal, as are evoked neurotransmission events. As in wild type, no photoreceptor defects, either morphological or functional, are seen in adult flies that have been exposed to 10 days of constant light stimulation.

      (Cherry et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4Rab7-Gal4-KO/Rab7T22N.UAS.Venus partially rescues Rab7Gal4-KO

      (Cherry et al., 2013)
      Comments

      Expression of Rab7T22N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Rab7.T:Disc\RFP fails to rescue the lethality seen in homozygous Rab7GAL4.T:Disc\RFP mutant flies. Occasional escapers are seen, but these die shortly after emergence.

      Expression of Rab7T22N.Scer\UAS.T:Avic\GFP-YFP.Venus under the control of Scer\GAL4Rab7.T:Disc\RFP partially rescues the progressive synaptic and neuronal degeneration seen in homozygous Rab7GAL4.T:Disc\RFP mutant eye clones after 10 days of constant light stimulation.

      (Cherry et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rab7T22N.Scer\UAS.T:Avic\GFP-YFP.Venus
      Rab7T22N.UAS.Venus
      Name Synonyms
      Secondary FlyBase IDs
        References (4)