FB2026_02 , released June 18, 2026
Allele: Dmel\Ino80psg25
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General Information
Symbol
Dmel\Ino80psg25
Species
D. melanogaster
Name
FlyBase ID
FBal0295026
Feature type
allele
Associated gene
Dmel\Ino80
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Neuman et al., 2014)
Progenitor genotype
Cytology
Description

Point mutation (G to A) in the splice donor site after the fourth exon. This results in the retention of a 26bp intron fragment, causing a frameshift that produces an in-frame stop codon immediately following the mutated donor site (the amino acid residue prior to the stop codon is also changed from Phe to Leu).

(Neuman et al., 2014)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:19,400,004..19,400,004 [-]
Nucleotide change:

G19400004A

Reported nucleotide change:

G?A

Comment:

Mutation in splice donor for fourth intron of Ino80

(Neuman et al., 2014)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Most homozygous and Ino80psg25/Df(3R)ED5942 animals die after head eversion, either as pupae or pharate adults. Approximately 15% of animals eclose, but they are sterile. Arrested mutant pupae and pharate adults appear morphologically normal.

      Ino80psg25/Df(3R)ED5942 animals have a persistant salivary gland phenotype (85.2% penetrance) at 24 hours after puparium formation (APF). Caspase activation appears to be delayed in the mutant salivary glands compared to wild type.

      Ino80psg25/Df(3R)ED5942 animals show a 2 hour delay in the timing of head eversion compared to controls. Those animals that eclose do so approximately one day later than controls.

      (Neuman et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of ftz-f1hs.PW is induced by a heat shock at 7 hours after puparium formation rescues the timing of head eversion in Ino80psg25/Df(3R)ED5942 animals to normal.

      (Neuman et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Ino80psg25/Df(3R)ED5942 is rescued by Ino80hs.PN

      (Neuman et al., 2014)

      Ino80psg25 is rescued by Ino80hs.PN

      (Neuman et al., 2014)
      Comments

      Leaky expression of Ino80hs.PN is able to rescue both the lethality and sterility observed in homozygous Ino80psg25 and Ino80psg25/Df(3R)ED5942 animals. In addition, the developmental delay seen in Ino80psg25/Df(3R)ED5942 animals is also rescued.

      (Neuman et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ino80psg25
      ino80psg25
      (Neuman et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)