FlyBase Allele Report: Dmel\Kat60[17A]

General Information

Symbol

Dmel\Kat60^17A

Species

D. melanogaster

Name

FlyBase ID

FBal0295261

Feature type

allele

Associated gene

Dmel\Kat60

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Mao et al., 2014)

amorphic allele - molecular evidence

(Mao et al., 2014)

Mutagen

ends-out gene targeting

(Mao et al., 2014)

Progenitor genotype

Cytology

Description

Deletion of sequence encoding 252 amino acid residues of Kat60, including the entire AAA domain in the C-terminus.

(Mao et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Mao et al., 2014)

abnormal neuroanatomy | third instar larval stage (with Df(3R)ED5138)

(Mao et al., 2014)

abnormal neurophysiology | third instar larval stage

(Mao et al., 2014)

lethal

(Mao et al., 2014)

lethal (with Df(3R)ED5138)

(Mao et al., 2014)

majority die during larval stage

(Mao et al., 2014)

some die during pupal stage

(Mao et al., 2014)

Phenotype Manifest In

dendritic tree

(Mao et al., 2014)

larval multidendritic class IV neuron

(Mao et al., 2014)

microtubule

(Mao et al., 2014)

NMJ bouton | third instar larval stage

(Mao et al., 2014)

NMJ bouton | third instar larval stage (with Df(3R)ED5138)

(Mao et al., 2014)

Detailed Description

Statement

Reference

80% of homozygotes die at larval stages and 20% live to the early pupal stage. The surviving mutant larvae appear sluggish.

Homozygous and Kat60^17A/Df(3R)ED5138 third instar larvae show defects at the neuromuscular junction (NMJ) compared to wild type: the total number of boutons and the number of satellite boutons are both significantly increased. In addition, a large number of enlarged vesicles up to 200nm in diameter are seen throughout the boutons in the NMJs of homozygous larvae.

The amplitude of the evoked excitatory junctional potential (EJP) at the NMJ is significantly decreased in homozygous larvae compared to wild type, while the amplitude and frequency of the spontaneous miniature EJP is unchanged. Quantal content is reduced.

Homozygous class IV dendritic arborization neurons show altered dendritic elaboration compared to wild type: the total dendritic length and the number of dendritic termini are significantly increased, while the total dendrite field area is normal.

Homozygous larval muscle cells have an unevenly distributed microtubule network and a significantly increased perinuclear microtubule density compared to wild-type cells.

(Mao et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Kat60^17A is rescued by Kat60^UAS.cMa

(Mao et al., 2014)

Kat60^17A is rescued by Kat60^UAS.cMa/Scer\GAL4^da.PU

(Mao et al., 2014)

Kat60^17A is rescued by Kat60^UAS.cMa/Scer\GAL4^elav.PU

(Mao et al., 2014)

Comments

The M{UAS-Kat60.M}ZH-51D insertion line effectively rescues the lethality of Kat60^17A animals, even in the absence of a Scer\GAL4 driver. The anatomical and electrophysiological defects at the neuromuscular junction which are seen in Kat60^17A third instar larvae are rescued by two copies of Kat60^Scer\UAS.cMa.

(Mao et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64116

Kat60^17A/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Kat60^17A

(Zheng et al., 2020)

Katanin 60^17A

(Mao et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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