FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\trkUASp.TagRFP,EGFP
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General Information
Symbol
Dmel\trkUASp.TagRFP,EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0295449
Feature type
allele
Associated gene
Dmel\trk
Associated Insertion(s)
Carried in Construct
P{UASp-TagRFP-trk-eGFP}
Key Links
Transgenic product class
loss_of_function_variant
(Henstridge et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
loss_of_function_variant
(Henstridge et al., 2014)
Mutagen
in vitro construct
(Henstridge et al., 2014)
Progenitor genotype
Carried in construct
P{UASp-TagRFP-trk-eGFP}
Cytology
Description

UASp regulatory sequences drive expression of wild-type trk that is tagged at the N-terminal end with TagRFP (the tag is located between residues D34-Y35, after the N-terminal signal peptide, and is flanked with a short SAGSAS linker peptide) and at the C-terminal end with EGFP. The presence of the C-terminal EGFP tag results in loss of function of the protein.

(Henstridge et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
trk
Tagged with
EGFP
TagRFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4VP16.nanos.UTR/trkUASp.TagRFP,EGFP fails to rescue trkΔ

      (Henstridge et al., 2014)
      Comments

      Maternal expression of trkScer\UAS.P\T.T:Zzzz\eqFP578-TagRFP,T:Avic\GFP-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fails to rescue the phenotype of embryos laid by trkΔ females.

      (Henstridge et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      trkScer\UAS.P\T.T:Equa\eqFP578-TagRFP,T:Avic\GFP-EGFP
      trkScer\UAS.P\T.T:Zzzz\eqFP578-TagRFP,T:Avic\GFP-EGFP
      trkScer\UAS.P\T.T:Zzzz\eqFP578-TagRFP-T,T:Avic\GFP-EGFP
      trkUASp.TagRFP,EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)