FlyBase Allele Report: Dmel\Nos[1]

General Information

Symbol

Dmel\Nos¹

Species

D. melanogaster

Name

FlyBase ID

FBal0295528

Feature type

allele

Associated gene

Dmel\Nos

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lacin et al., 2014)

Mutagen

ethyl methanesulfonate

(Lacin et al., 2014)

Progenitor genotype

Cytology

Description

Amino acid coordinates refer to the Nos A isoform.

(Lacin et al., 2014)

Amino acid replacement: W591term.

(Lacin et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,816,207..10,816,207 [-]

Nucleotide change:

G10816207A

Amino acid change:

W591term | Nos-PA; W591term | Nos-PF; W340term | Nos-PK

Reported amino acid change:

W591term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lacin et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of epilepsy

CEA

(Giachello et al., 2021)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number (with Nos^Δ15)

(Cho et al., 2024)

abnormal neuroanatomy | P-stage (with Nos^C)

(Rabinovich et al., 2016)

abnormal neuroanatomy | P-stage (with Nos^Δ15)

(Rabinovich et al., 2016)

fertile

(Lacin et al., 2014)

fertile (with Nos^Δ15)

(Lacin et al., 2014)

viable

(Lacin et al., 2014)

viable (with Nos^Δ15)

(Lacin et al., 2014)

Phenotype Manifest In

adult mushroom body gamma-lobe | P-stage (with Nos^C)

(Rabinovich et al., 2016)

adult mushroom body gamma-lobe | P-stage (with Nos^Δ15)

(Rabinovich et al., 2016)

axon | P-stage (with Nos^C)

(Rabinovich et al., 2016)

axon | P-stage (with Nos^Δ15)

(Rabinovich et al., 2016)

embryonic/larval lymph gland (with Nos^Δ15)

(Cho et al., 2024)

Detailed Description

Statement

Reference

A subset of Nos^C/Nos¹ or Nos^Δ15/Nos¹ mutants display pruning defects and/or precocious regrowth of axons in the mushroom body gamma-lobe, as compared to Nos^Δ15/+, Nos¹/+ or Nos^C/+ controls.

(Rabinovich et al., 2016)

Homozygous Nos¹ mutant larvae exhibit normal development and developmental timing, and give rise to viable, fertile and morphologically wild type adult flies. CNS development also appears normal.

Nos¹/Nos^Δ15 mutant larvae exhibit normal development and developmental timing, and give rise to viable, fertile and morphologically wild type adult flies. CNS development also appears normal and there is no overt effect on taste or feeding behavior compared to controls. Nos¹/Nos^Δ15 mutant flies exhibit locomotor defects; flies walk considerably more, and stop considerably less, than control flies.

(Lacin et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

56822

w¹¹¹⁸; Nos¹/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nos¹

(Cho et al., 2024, Giachello et al., 2021, Song et al., 2019, Lacin et al., 2014)

dNOS¹

(Rabinovich et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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