In-vivo exchange between the FRT5 site upstream of the bcd open reading frame and an FRT5 site in a donor construct has been used to replace the UAS regulatory sequences present in bcdUAS.FRT5.FRT2.Tag:HA with lexAop regulatory sequences.
wing pouch, with Ecol\lexALHG.dpp
Expression of bcdEcol\lexAop.FRT5.FRT2.T:Ivir\HA1 under the control of Ecol\lexAdpp.T:Scer\GAL4-H,T:Scer\GAL4-GAD results in a pronounced fold in the wing pouch domain of the wing disc.