FlyBase Allele Report: Hsap\RELA[AD.R20C11]

General Information

Symbol

Hsap\RELA^AD.R20C11

Species

H. sapiens

Name

FlyBase ID

FBal0295719

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R20C11-p65.AD}

PBac{R20C11-p65.AD}

Also Known As

R20C11-p65ADZp, R20C11-AD

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Chen et al., 2014)

Progenitor genotype

Carried in construct

P{R20C11-p65.AD}

PBac{R20C11-p65.AD}

Cytology

Description

The GMR20C11 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR20C11

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

centrifugal neuron C2

Comment: when combined with P{R25B02-GAL4.DBD}

when combined with P{R48D11-GAL4.DBD}

(Nern et al., 2015, Tuthill et al., 2013)

centrifugal neuron C3

Comment: when combined with P{R48D11-GAL4.DBD}

(Nern et al., 2015, Tuthill et al., 2013)

columnar neuron T1 | subset

Comment: when combined with P{R48D11-GAL4.DBD}

(Tuthill et al., 2013)

columnar neuron T1

Comment: when combined with P{R30F10-GAL4.DBD}

(Nern et al., 2015)

centrifugal neuron C2 | subset

Comment: when combined with P{R25B02-GAL4.DBD} (combination referred to as 'C2a-Gal4')

(Yuan et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Hsap\MAPT^{P301L.UAS.0N4R}, Scer\GAL4^DBD.R48D11

(Praschberger et al., 2023)

Phenotype Manifest In

adult optic lobe, with Hsap\MAPT^{P301L.UAS.0N4R}, Scer\GAL4^DBD.R48D11

(Praschberger et al., 2023)

optic lobe centrifugal columnar neuron | adult stage, with Hsap\MAPT^{P301L.UAS.0N4R}, Scer\GAL4^DBD.R48D11

(Praschberger et al., 2023)

varicosity | adult stage, with Hsap\MAPT^{P301L.UAS.0N4R}, Scer\GAL4^DBD.R48D11

(Praschberger et al., 2023)

Detailed Description

Statement

Reference

Activation of C2 lamina neurons, using Hsap\RELA^{AD.R20C11.T:Zzzz\Zip+}, Scer\GAL4^{DBD.R25B02.T:Zzzz\Zip-} and TrpA1^Scer\UAS.cUa, increases steering responses to regressive motion and decreases responses to progressive motion.

Silencing of C2 + C3 lamina neurons, using Hsap\RELA^{AD.R20C11.T:Zzzz\Zip+}, Scer\GAL4^{DBD.R48D11.T:Zzzz\Zip-} and Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, dramatically shifts fly responses to all regressive motion stimuli, such that clockwise regressive motion causes flies to turn counterclockwise. Behavioural responses to progressive motion are unaffected.

Silencing of C2 lamina neurons, using Hsap\RELA^{AD.R20C11.T:Zzzz\Zip+}, Scer\GAL4^{DBD.R25B02.T:Zzzz\Zip-} and Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, abolishes reverse-optomotor inversion at high speeds.

Silencing of C2 lamina neurons, using Hsap\RELA^{AD.R20C11.T:Zzzz\Zip+}, Scer\GAL4^{DBD.R25B02.T:Zzzz\Zip-} and Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, affects the ability of flies to distinguish small contrast differences at low test speeds, while behaviour at higher test speeds remains normal.

(Tuthill et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions