Silencing of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4^Tk and Ecol\lexA^{fru.P1.T:Hsim\VP16}) reduces levels of male-male aggression compared to controls, although the level of baseline aggression between different controls varies. Ecol\lexA^{fru.P1.T:Hsim\VP16} drives expression of Scer\FLP1^{L.8xEcol\lexAop}, which results in excision of the Scer\FRT cassette from Hsap\KCNJ2^{Scer\FRT.Stop.Scer\UAS.T:Avic\GFP-EGFP} enabling these cells to express Hsap\KCNJ2 under the control of Scer\GAL4^Tk. The males copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged. Silencing of Tk-GAL4[Fru[M] neurons does not consistently influence the frequency of unilateral wing extensions towards other males in comparison with control strains.