FlyBase Allele Report: Dmel\temp[A]

General Information

Symbol

Dmel\temp^A

Species

D. melanogaster

Name

FlyBase ID

FBal0295877

Feature type

allele

Associated gene

Dmel\temp

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Graves et al., 2020, Charng et al., 2014, Yamamoto et al., 2013-)

Progenitor genotype

Cytology

Description

Amino acid replacement: K14term.

(Graves et al., 2020, Charng et al., 2014, Yamamoto et al., 2013-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:2,261,997..2,261,997 [-]

Nucleotide change:

A2261997T

Amino acid change:

K14term | temp-PA; K14term | temp-PB

Reported amino acid change:

K14term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Charng et al., 2014, Haelterman et al., 2014, Yamamoto et al., 2013-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Charng et al., 2014)

some die during second instar larval stage

(Charng et al., 2014)

some die during third instar larval stage

(Charng et al., 2014)

visible | somatic clone

(Charng et al., 2014)

Phenotype Manifest In

chaeta | somatic clone

(Charng et al., 2014)

external sensory organ precursor cell I | somatic clone

(Charng et al., 2014)

mesonotum | somatic clone

(Charng et al., 2014)

Detailed Description

Statement

Reference

Homozygous clones results in a strong balding phenotype in the adult notum. At 12 hours after puparium formation, the density of sensory organ precursor cells is higher in the mutant clone tissue than in neighbouring wild-type tissue.

(Charng et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

temp^A has mesonotum | somatic clone phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Hsap\PTAR1^UAS.Tag:FLAG

(Charng et al., 2014)

temp^A has chaeta | somatic clone phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Hsap\PTAR1^UAS.Tag:FLAG

(Charng et al., 2014)

temp^A has external sensory organ precursor cell I | somatic clone phenotype, suppressible | somatic clone by Scer\GAL4^Act.PU/Hsap\PTAR1^UAS.Tag:FLAG

(Charng et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PTAR1^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act.PU in homozygous temp^A clones in the adult notum rescues the balding phenotype normally seen in these clones.

(Charng et al., 2014)

Complementation and Rescue Data

Fails to complement

temp^G0144

(Charng et al., 2014)

temp^G0144

(Yamamoto et al., 2013-)

Rescued by

temp^A is rescued by temp^Tag:HA

(Charng et al., 2014)

temp^A is rescued by temp^mCherry

(Charng et al., 2014)

temp^A is rescued by Scer\GAL4^Act.PU/temp^UAS.Tag:HA

(Charng et al., 2014)

Comments

temp^T:Ivir\HA1 and temp^{T:Disc\RFP-mCherry} each rescue the lethality of temp^A homozygotes and the notum balding phenotype of temp^A homozygous clones.

Expression of temp^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU rescues the homozygous lethal phenotype and the homozygous clone notum balding phenotype of temp^A.

(Charng et al., 2014)

Rescued by: Dp(1;3)DC039.

(Yamamoto et al., 2013-)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

57094

y¹ temp^A w^* P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

XGZK109

(Graves et al., 2020)

XM12A

(Graves et al., 2020)

temp^A

(Graves et al., 2020, Charng et al., 2014, Yamamoto et al., 2013-)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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