FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\MetGFP.FPTB
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General Information
Symbol
Dmel\MetGFP.FPTB
Species
D. melanogaster
Name
FlyBase ID
FBal0295939
Feature type
allele
Associated gene
Dmel\Met
Associated Insertion(s)
Carried in Construct
PBac{Met-GFP.FPTB}
Key Links
Transgenic product class
genomic_DNA
(Victorsen and White, 2014.5.13)
wild_type
(Victorsen and White, 2014.5.13)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Victorsen and White, 2014.5.13)
wild_type
(Victorsen and White, 2014.5.13)
Mutagen
in vitro construct
(Victorsen and White, 2014.5.13)
Progenitor genotype
Carried in construct
PBac{Met-GFP.FPTB}
Cytology
Description

Genomic fragment including the Met gene, from BAC clone CH321-28O05. A sfGFP-Tag:FLAG-Tag:CS(PSP)-Tag:CS(TEVp)-Tag:BLRP tag cassette has been introduced at the C-terminal end of the Met gene, such that protein isoforms A and B are tagged.

(Victorsen and White, 2014.5.13)
Allele components
Component
Use(s)
Regulatory region(s)
Met
Encoded product / tool
Met
Tagged with
sfGFP
Tag:BLRP
Tag:CS(PSP)
Tag:CS(TEVp)
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      wandering third instar larval stage
      embryonic/larval fat body
      (Liu et al., 2018)
      embryonic/larval salivary gland
      (Liu et al., 2018)
      prothoracic gland
      (Liu et al., 2018)
      adult midgut progenitor cell
      (Liu et al., 2018)
      Additional Information
      Statement
      Reference

      Met-GFP is detected in fat body, salivary gland, prothoracic gland and adult midgut progenitor cells in early wandering third instar larvae but not in the larval midgut cells.

      (Liu et al., 2018)
       
      Marker for
      Reflects expression of
      Met
      (Liu et al., 2018)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      MetGFP.FPTB
      MetT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP
      MetsfGFP,Tag:FLAG,Tag:CS(PSP),Tag:CS(TEVp),Tag:BLRP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)