FlyBase Recombinant Construct Report: PBac{Met-GFP.FPTB}

Genomic fragment including the Met gene, from BAC clone CH321-28O05. A sfGFP-Tag:FLAG-Tag:CS(PSP)-Tag:CS(TEVp)-Tag:BLRP tag cassette has been introduced at the C-terminal end of the Met gene, such that protein isoforms A and B are tagged.

(Victorsen and White, 2014.5.13)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Met^GFP.FPTB

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval fat body

(Liu et al., 2018)

embryonic/larval salivary gland

(Liu et al., 2018)

prothoracic gland

(Liu et al., 2018)

adult midgut progenitor cell

(Liu et al., 2018)

Additional Information

Statement

Reference

Met-GFP is detected in fat body, salivary gland, prothoracic gland and adult midgut progenitor cells in early wandering third instar larvae but not in the larval midgut cells.

(Liu et al., 2018)

Marker for

Reflects expression of

Met

(Liu et al., 2018)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

56190

y¹ w^*; PBac{Met-GFP.FPTB}VK00031

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Met-GFP

(Liu et al., 2018)

PBac{Met-GFP.FPTB}

(Victorsen and White, 2014.5.13, FlyBase, 1992-)

Secondary FlyBase IDs

References (4)

Report Sections

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