FlyBase Allele Report: Dmel\PNUTS[13B]

General Information

Symbol

Dmel\PNUTS^13B

Species

D. melanogaster

Name

FlyBase ID

FBal0296061

Feature type

allele

Associated gene

Dmel\PNUTS

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Ciurciu et al., 2013)

Progenitor genotype

PNUTS^KG00572

(Ciurciu et al., 2013)

Cytology

Description

Imprecise excision of P{SUPor-P}PNUTS^KG00572 generates a deletion in PNUTS that removes the entire PNUTS coding region, including the translation start site.

(Ciurciu et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:870,816..876,798 [+]

Comment:

5984 bp deletion resulting from the imprecise excision of P{SUPor-P}PNUTS^KG00572, which removes the entire PNUTS coding region. Coordinates inferred from reported boundary sequences.

(Ciurciu et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye

(Ciurciu et al., 2013)

nucleus | female limited, with PNUTS^ED.EGFP

(Duncalf et al., 2025)

nucleus | female limited, with PNUTS^EED.EGFP

(Duncalf et al., 2025)

nucleus | female limited | somatic clone - tissue specific, with PNUTS^FRT.EGFP

(Duncalf et al., 2025)

nurse cell, with PNUTS^ED.EGFP

(Duncalf et al., 2025)

nurse cell, with PNUTS^EED.EGFP

(Duncalf et al., 2025)

nurse cell | germline clone | somatic clone - tissue specific, with PNUTS^FRT.EGFP

(Duncalf et al., 2025)

ommatidium

(Ciurciu et al., 2013)

Detailed Description

Statement

Reference

Homozygous PNUTS^13B animals develop to 1st instar larvae but then die in the ensuing 8 days without further growth and development.

Eyes of heterozygous PNUTS^13B flies resemble wild-type. Flies with eyes homozygous for PNUTS^13B are reduced in size, with fewer and poorly organised ommatidia.

Induction of PNUTS^13B homozygous somatic clones in 1st instar larvae does not generate clonal patches, while wild-type twin-spot clones generate a large clonal patch.

(Ciurciu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

PNUTS^13B has lethal - all die before end of larval stage | recessive phenotype, suppressible by Tox4^EGFP

(Duncalf et al., 2025)

PNUTS^13B has abnormal cell growth phenotype, suppressible | partially by RpL27A[+]/RpL27A¹

(Ciurciu et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

PNUTS^13B clones induced in a RpL27A¹ heterozygous background span the entire wing disc epithelium, indicating that they are not eliminated, as in a wild-type background. However, these mutant clones colonised a significantly smaller area of RpL27A¹/+ discs compared with wild-type clones, indicating that they are still growth impaired.

(Ciurciu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

PNUTS^13B is rescued by PNUTS^ED.EGFP

(Duncalf et al., 2025)

PNUTS^13B is rescued by PNUTS^EED.EGFP

(Duncalf et al., 2025)

PNUTS^13B/PNUTS^9B is rescued by PNUTS^+t9.1.cCa

(Ciurciu et al., 2013)