FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\wekUAS.ORF.GW.Tag:HA
Open Close
General Information
Symbol
Dmel\wekUAS.ORF.GW.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0297239
Feature type
allele
Associated gene
Dmel\wek
Associated Insertion(s)
Carried in Construct
M{UAS-wek.ORF.3xHA.GW}
Key Links
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
in vitro construct
(Bischof and FlyORF project members, 2014.6.20)
Progenitor genotype
Carried in construct
M{UAS-wek.ORF.3xHA.GW}
Cytology
Description

UAS regulatory sequences drive expression of a full-length wek open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.

(Bischof and FlyORF project members, 2014.6.20)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wek
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4elav.PU results in increased number of apoptotic (Dcp-1-positive) cells in pupal ventral nerve cord (VNC) and decrease in the number of eve-positive neurons both in embryos and in pupal VNC, as compared to controls. Scer\GAL4Myd88-NP6394-driven expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 also leads to a moderate loss of eve+ neurons and cells within the driver's expression domain (assayed using a nuclear histone-reporter) in the pupal abdominal VNC.

      (Foldi et al., 2017)

      Expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in male flies with severe wing size and patterning defects.

      Expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with weak wing size and patterning defects.

      (Schertel et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing wekScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4elav.PU is suppressed by co-expression of Ect4VDRC.cUa.

      The decrease in the number of cells within the Scer\GAL4Myd88-NP6394 driver's expression domain in the pupal ventral nerve cord (assayed using a nuclear histone-reporter) observed upon expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 is exacerbated further by co-expression of Myd88VDRC.cUa.

      (Foldi et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav.PU/wekUAS.ORF.GW.Tag:HA rescues wekEX14/Df(2L)BSC690

      (Foldi et al., 2017)
      Comments

      The reduced level of cell death (decreased number of Dcp-1-positive cells) observed in the ventral nerve cord (VNC) of wekEX14/Df(2L)BSC690 and the increase in cell death induced by Scer\GAL4elav.PU-driven expression of wekScer\UAS.ORF.GW.T:Ivir\HA1 can both be mitigated by combining the two manipulations together: The number of apoptotic cells in the VNC of wekEX14/Df(2L)BSC690 mutants expressing wekScer\UAS.ORF.GW.T:Ivir\HA1 is intermediate to each of the two perturbations and higher than in controls.

      (Foldi et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wekScer\UAS.ORF.GW.T:Ivir\HA1
      wekUAS.ORF.GW.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (4)