FlyBase Allele Report: Dmel\wake[Δ]

General Information

Symbol

Dmel\wake^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0298780

Feature type

allele

Associated gene

Dmel\wake

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Mauri et al., 2014)

Progenitor genotype

PBac{RB}Gclm^e01794

(Mauri et al., 2014)

P{XP}d07120

(Mauri et al., 2014)

Caused by aberration

Df(3R)wake^Δ

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Mauri et al., 2014)

lethal - all die before end of P-stage

(Mauri et al., 2014)

Phenotype Manifest In

larval brain

(Mauri et al., 2014)

mitotic spindle

(Mauri et al., 2014)

neuroblast

(Mauri et al., 2014)

sensory mother cell

(Mauri et al., 2014)

Detailed Description

Statement

Reference

The few wake^Δ mutant escapers that are seen display severe locomotion defects and die within hours of eclosion. Defects are seen in the establishment of polarity in mitotic sensory organ precursor (SOP) cells prior to anaphase, but many of these defects are corrected in later mitosis. Spindle positioning is also disrupted.

A strong loss of neuroblasts is seen in homozygous wake^Δ mutant larval brains compared to heterozygous controls. The number of neurons is not affected. The number of optic lobe neuroblasts is unaffected and the overall structure of the brain is normal, although homozygous wake^Δ mutant brains are slightly smaller on average. The remaining neuroblasts are smaller than in wild type. Defects are seen in polarity establishment in mitotic cells prior to anaphase, but many of these defects are corrected in later mitosis.

(Mauri et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

dlg1^sw, wake^Δ has neoplasia phenotype

(Mauri et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

dlg1^sw, wake^Δ has larval brain phenotype

(Mauri et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

In contrast to either allele alone, the brains of dlg1^sw, wake^Δ double mutant larvae contain massive tumors.

In contrast to either allele alone, the brains of dlg1², wake^Δ double mutant larvae contain massive tumors.

(Mauri et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

wake^Δ is rescued by wake^UAS.GFP

(Mauri et al., 2014)

wake^Δ is rescued by wake^{+tCH322-118J18}

(Mauri et al., 2014)

Comments

Expression of wake^{+tCH322-118J18} rescues the neuroblast loss seen in the brains of wake^Δ mutant larvae.

Expression of wake^{Scer\UAS.T:Avic\GFP} in all neuroblasts under the control of Scer\GAL4^insc-Mz1407 rescues the neuroblast loss seen in the brains of wake^Δ mutant larvae.

(Mauri et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

bnd^Δ

(Mauri et al., 2014)

wake^Δ

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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