FB2026_02 , released June 18, 2026
Allele: Dmel\shiTag:FLAG,Tag:CALI(4C)
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General Information
Symbol
Dmel\shiTag:FLAG,Tag:CALI(4C)
Species
D. melanogaster
Name
FlyBase ID
FBal0298853
Feature type
allele
Associated gene
Dmel\shi
Associated Insertion(s)
Carried in Construct
PBac{shi-4C}
Key Links
Transgenic product class
genomic_DNA
(Kasprowicz et al., 2014)
wild_type
(Kasprowicz et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Kasprowicz et al., 2014)
wild_type
(Kasprowicz et al., 2014)
Mutagen
in vitro construct
(Kasprowicz et al., 2014)
Progenitor genotype
Carried in construct
PBac{shi-4C}
Cytology
Description

Genomic fragment containing shi. A Tag:FLAG-Tag:CALI(4C) tag has been inserted into the middle domain of the shi gene. The presence of the Tag:CALI(4C) tag means that FlAsH-mediated fluorescein-assisted light inactivation can be used to inactivate the protein produced.

(Kasprowicz et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
shi
Encoded product / tool
shi
Tagged with
Tag:CALI(4C)
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inactivation of the shiT:Zzzz\FLAG,T:Zzzz\4C protein using FlAsH-mediated fluorescein-assisted light inactivation in shi12.12B flies carrying shiT:Zzzz\FLAG,T:Zzzz\4C results in a fast drop in the EJP amplitude at intense (10Hz) stimulation.

      Stimulation of boutons in which shi function has been inactivated (using FlAsH-mediated fluorescein-assisted light inactivation to photo-inactivate shiT:Zzzz\FLAG,T:Zzzz\4C in shi12.12B flies carrying shiT:Zzzz\FLAG,T:Zzzz\4C) results in accumulation of large membraneous profiles, some of which are seen to be connected to the presynaptic membrane. In addition, there is an almost complete lack of normal-size synaptic vesicles in the synaptic terminal.

      shi1/Y ; shiT:Zzzz\FLAG,T:Zzzz\4C animals at the restrictive temperature (without photo-inactivating shiT:Zzzz\FLAG,T:Zzzz\4C) show a block in membrane uptake upon stimulation of boutons compared to controls at 22[o]C and show accumulation of invaginated pits. In shi1/Y ; shiT:Zzzz\FLAG,T:Zzzz\4C animals at the permissive temperature in which shiT:Zzzz\FLAG,T:Zzzz\4C has been photo-inactivated, large membrane inclusions are seen in the boutons after stimulation. Membrane accumulations in stimulated boutons are also seen at the restrictive temperature in shi1/Y ; shiT:Zzzz\FLAG,T:Zzzz\4C animals in which shiT:Zzzz\FLAG,T:Zzzz\4C has been photo-inactivated, but accumulation of invaginated pits is not seen in this case.

      (Kasprowicz et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      shiTag:FLAG,Tag:CALI(4C) rescues shi12.12B

      (Kasprowicz et al., 2014)
      Comments

      shiT:Zzzz\FLAG,T:Zzzz\4C fully rescues the lethality of shi12.12B mutants, and the rescued animals have no obvious behavioural, developmental or electrophysiological defects.

      (Kasprowicz et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shiT:Zzzz\FLAG,T:Zzzz\4C
      shiTag:FLAG,Tag:CALI(4C)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)