FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\EndoATag:CALI(4C)
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General Information
Symbol
Dmel\EndoATag:CALI(4C)
Species
D. melanogaster
Name
FlyBase ID
FBal0298854
Feature type
allele
Associated gene
Dmel\EndoA
Associated Insertion(s)
Carried in Construct
P{EndoA-4C}
Key Links
Transgenic product class
wild_type
(Kasprowicz et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Kasprowicz et al., 2014)
Mutagen
in vitro construct
(Kasprowicz et al., 2014)
Progenitor genotype
Carried in construct
P{EndoA-4C}
Cytology
Description

Contains EndoA with a Tag:CALI(4C) tag inserted between the BAR and SH3 domains. The presence of the Tag:CALI(4C) tag means that FlAsH-mediated fluorescein-assisted light inactivation can be used to inactivate the protein produced.

(Kasprowicz et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
EndoA
Encoded product / tool
EndoA
Tagged with
Tag:CALI(4C)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      EndoAΔ4 heterozygotes containing a copy of EndoAT:Zzzz\4C are viable. In these individuals, light-induced inactivation of EndoAT:Zzzz\4C at the presynaptic region of third instar larval neuromuscular junctions results in significant decreases in the local level of endocytosis and in the local formation of autophagosomes (detected as fluorescent Atg8 puncta in confocal microscopy images) in response to electric-stimulation, as compared to 'non-inactivated' and wild-type controls.

      (Soukup et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      EndoAT:Zzzz\4C
      EndoATag:CALI(4C)
      endoA4C
      (Soukup et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)