UAS regulatory sequences drive expression of vas carrying a point mutation (DQAD) in its DEAD box and fused downstream of GFP.
Scer\GAL4VP16.nanos.UTR/vasDQAD.UAS.GFP fails to rescue vas1/vas3
Expression of vasDQAD.Scer\UAS.T:Avic\GFP using Scer\GAL4nos.UTR.T:Hsim\VP16 fails to rescue the infertility phenotype of vas1/vas3 mutants.