FlyBase Allele Report: Zzzz\poly-GR[PO-36.UAS]

General Information

Symbol

Zzzz\poly-GR^PO-36.UAS

Species

a. artificial

Name

FlyBase ID

FBal0300894

Feature type

allele

Associated gene

Zzzz\poly-GR

Associated Insertion(s)

Carried in Construct

P{UAS-poly-GR.PO-36}

Also Known As

UAS-GR36, GR36, UAS-(GR)36, (GR)36, GR-36

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Mizielinska et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-poly-GR.PO-36}

Cytology

Description

UAS regulatory sequences drive expression of 36 pure repeats of a "protein-only" version of Zzzz\poly-GR using alternative codons to those found within the GGGGCC repeat.

(Mizielinska et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Zzzz\poly-GR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis

CEA

(Hautbergue et al., 2017, Mizielinska et al., 2014)

model of C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

CEA

(Milioto et al., 2024, Li et al., 2023, Morón-Oset et al., 2023, Rossi et al., 2023, Xu et al., 2023, Farrugia et al., 2022, Fumagalli et al., 2021, Park et al., 2021, Park et al., 2020, Ramesh et al., 2020, Mordes et al., 2018, Xu and Xu, 2018)

model of frontotemporal dementia

CEA

(Mizielinska et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis

is NOT ameliorated by SF2^GD12097

(Hautbergue et al., 2017)

model of C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

is exacerbated by Mp^HMJ21668

(Milioto et al., 2024)

is exacerbated by Mp^JF02929

(Milioto et al., 2024)

is exacerbated by daw^HMJ03135

(Milioto et al., 2024)

is exacerbated by daw^HMS01110

(Milioto et al., 2024)

is exacerbated by Khc²⁷

(Fumagalli et al., 2021)

is exacerbated by Khc⁸

(Fumagalli et al., 2021)

is exacerbated by Adck1^NIG.3608R

(Park et al., 2021)

is exacerbated by Arf4^NIG.11027R

(Park et al., 2021)

is exacerbated by Brf^NIG.4155R

(Park et al., 2021)

is exacerbated by CG11414^HMC06381

(Park et al., 2021)

is exacerbated by CG11414^HMJ23120

(Park et al., 2021)

is exacerbated by CG2931^NIG.2931R

(Park et al., 2021)

is exacerbated by CG31643^NIG.31643R

(Park et al., 2021)

is exacerbated by CG5776^HMS02672

(Park et al., 2021)

is exacerbated by CG6013^NIG.6013R

(Park et al., 2021)

is exacerbated by CG8635^NIG.8635R

(Park et al., 2021)

is exacerbated by Cand1^NIG.5366R

(Park et al., 2021)

is exacerbated by Capr^NIG.18811R

(Park et al., 2021)

is exacerbated by Clbn^GD7403

(Park et al., 2021)

is exacerbated by Rack1^GD12135

(Park et al., 2021)

is exacerbated by Rack1^GL00637

(Park et al., 2021)

is exacerbated by Clbn^HMJ23825

(Park et al., 2021)

is exacerbated by Rack1^NIG.7111R

(Park et al., 2021)

is exacerbated by Hsp110^NIG.6603R

(Park et al., 2021)

is exacerbated by Mtr4^NIG.4152R

(Park et al., 2021)

is exacerbated by RpS27A^NIG.5271R

(Park et al., 2021)

is exacerbated by Nopp140^NIG.7421R

(Park et al., 2021)

is exacerbated by Npl4^HMJ21721

(Park et al., 2021)

is exacerbated by SPoCk^NIG.32451R

(Park et al., 2021)

is exacerbated by Smg5^NIG.8954R

(Park et al., 2021)

is exacerbated by P5CS^NIG.7470R

(Park et al., 2021)

is exacerbated by Polr2D^NIG.31237R

(Park et al., 2021)

is exacerbated by RNaseZ^NIG.3298R

(Park et al., 2021)

is exacerbated by Rab14^NIG.4212R

(Park et al., 2021)

is exacerbated by Smg6^NIG.6369R

(Park et al., 2021)

is exacerbated by Smn^NIG.16725R

(Park et al., 2021)

is exacerbated by Tbcc^NIG.31961R

(Park et al., 2021)

is exacerbated by TER94^JF03402

(Park et al., 2021)

is exacerbated by Upf3^NIG.11184R

(Park et al., 2021)

is exacerbated by eIF2Bβ^NIG.2677R

(Park et al., 2021)

is exacerbated by eIF3d1^NIG.10161R

(Park et al., 2021)

is exacerbated by eIF4E1^NIG.4035R

(Park et al., 2021)

is exacerbated by hoip^NIG.3949R

(Park et al., 2021)

is exacerbated by hyd^NIG.9484R

(Park et al., 2021)

is exacerbated by pix^JF01190

(Park et al., 2021)

is exacerbated by rig^NIG.11171R

(Park et al., 2021)

is exacerbated by smid^NIG.8571R

(Park et al., 2021)

is ameliorated by bumpel^{UAS.ORF.GW.Tag:HA}

(Xu et al., 2023)

is exacerbated by Hsf^+t8

(Mordes et al., 2018)

is exacerbated by ArfGAP1^HMJ30212

(Rossi et al., 2023)

is exacerbated by ergic53^HMC03809

(Rossi et al., 2023)

is exacerbated by Hsap\TARDBP^{UAS.Tag:HA,RFP(Unk)}

(Li et al., 2023)

is exacerbated by Hsap\APP^{695.UAS.Tag:MYC}

(Li et al., 2023)

is exacerbated by Hsap\FUS^{WT.UAS.RFP(Unk),Tag:HA}

(Li et al., 2023)

exacerbates spinocerebellar ataxia 1

modeled by Hsap\ATXN1^82Q.UAS

(Tazelaar et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PS

(Fujino et al., 2023)

abnormal eye color | adult stage, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^da.G32

(Hautbergue et al., 2017)

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Goodman et al., 2019)

decreased size | adult stage, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023)

increased cell death | adult stage, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020)

melanotic necrosis | adult stage, with Scer\GAL4^GMR.PU

(Ortega et al., 2023)

partially lethal, with Scer\GAL4^GMR.PU

(Mizielinska et al., 2014)

partially lethal - majority live, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023)

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Morón-Oset et al., 2023, Xu et al., 2023)

visible, with Scer\GAL4^GMR.PU

(Mizielinska et al., 2014)

visible | adult stage, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020, Goodman et al., 2019, Hautbergue et al., 2017)

visible | adult stage, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023, Ortega et al., 2023, Park et al., 2021, Tazelaar et al., 2020)

visible | adult stage, with Scer\GAL4^GMR.PY

(Villa et al., 2024)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Ramesh et al., 2020, Goodman et al., 2019)

eye, with Scer\GAL4^GMR.PS

(Fujino et al., 2023)

eye, with Scer\GAL4^GMR.PU

(Morón-Oset et al., 2023, Ortega et al., 2023, Park et al., 2021, Tazelaar et al., 2020, Mizielinska et al., 2014)

eye, with Scer\GAL4^GMR.PY

(Villa et al., 2024)

eye | adult stage, with Scer\GAL4^GMR.PF

(Hautbergue et al., 2017)

retina, with Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Detailed Description

Statement

Reference

The expression of Zzzz\poly-GR^PO-36.UAS under the control of Scer\GAL4^GMR.PU induces a eye degeneration phenotype

(Park et al., 2021)

The expression of Zzzz\poly-GR^PO-36.UAS under the control of Scer\GAL4^GMR.PF external eye degeneration including necrotic patches.

(Ramesh et al., 2020)

Expression of Zzzz\poly-GR^PO-36.UAS under the control of Scer\GAL4^GMR.PU leads to rough eye phenotype (with eye depigmentation and necrotic spots).

(Tazelaar et al., 2020)

Adults expressing Zzzz\poly-GR^PO-36.UAS under the control of Scer\GAL4^GMR.PF show mild pigment loss and dramatic loss of retinal tissue.

(Goodman et al., 2019)

The expression of Zzzz\poly-GR^{PO-36.Scer\UAS} leads to a severe rough eye phenotype (Scer\GAL4^GMR.PF-driven expression) and to a severe decrease in adult locomotor activity in climbing assays (Scer\GAL4^da.G32-driven expression), as compared to controls.

(Hautbergue et al., 2017)

Expression of Zzzz\poly-GR^{PO-36.Scer\UAS} using Scer\GAL4^GMR.PU causes eye degeneration and lethality.

(Mizielinska et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Otud6^C183A/Otud6[+]

(Villa et al., 2024)

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Otud6^KK105705, Scer\GAL4^GMR.PY

(Villa et al., 2024)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by CG11414^HMC06381, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Clbn^HMJ23825, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by CG11414^HMJ23120, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Ltn1^G9156, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Nulp1^G6757, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Tazelaar et al., 2020)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by eIF4B^HMS04503/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has abnormal size | adult stage phenotype, enhanceable by eIF4B^HMS04503/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, enhanceable by eIF4H1^HMS04504/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has abnormal size | adult stage phenotype, enhanceable by eIF4H1^HMS04504/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, non-enhanceable by Otud6^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PY

(Villa et al., 2024)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, non-enhanceable by Hsap\ATXN1^B.2Q.UAS, Scer\GAL4^GMR.PU

(Tazelaar et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^{elav.Switch.PO}, Zzzz\poly-GR^PO-36.UAS has short lived | RU486 conditional phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/bumpel^{UAS.ORF.GW.Tag:HA}

(Xu et al., 2023)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, non-suppressible by Otud6^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PY

(Villa et al., 2024)

Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-36.UAS has abnormal eye color phenotype, non-suppressible by Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Fujino et al., 2023)

Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-36.UAS has abnormal eye color phenotype, non-suppressible by Hsap\FUS^RRMmut.UAS, Scer\GAL4^GMR.PS

(Fujino et al., 2023)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, non-suppressible by Hsap\ATXN1^B.2Q.UAS, Scer\GAL4^GMR.PU

(Tazelaar et al., 2020)

Scer\GAL4^da.G32, Zzzz\poly-GR^PO-36.UAS has abnormal locomotor behavior | adult stage phenotype, non-suppressible by SF2^GD12097, Scer\GAL4^da.G32

(Hautbergue et al., 2017)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has visible | adult stage phenotype, non-suppressible by SF2^GD12097, Scer\GAL4^GMR.PF

(Hautbergue et al., 2017)

Other

Statement

Reference

Nulp1^G6757, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has increased cell death | adult stage phenotype

(Park et al., 2021)

Nulp1^G6757, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has decreased size | adult stage phenotype

(Park et al., 2021)

CG11414^HMC06381, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has increased cell death | adult stage phenotype

(Park et al., 2021)

CG11414^HMC06381, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has decreased size | adult stage phenotype

(Park et al., 2021)

CG11414^HMJ23120, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has increased cell death | adult stage phenotype

(Park et al., 2021)

CG11414^HMJ23120, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has decreased size | adult stage phenotype

(Park et al., 2021)

Clbn^HMJ23825, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has increased cell death | adult stage phenotype

(Park et al., 2021)

Clbn^HMJ23825, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has decreased size | adult stage phenotype

(Park et al., 2021)

Ltn1^G9156, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has increased cell death | adult stage phenotype

(Park et al., 2021)

Ltn1^G9156, Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has decreased size | adult stage phenotype

(Park et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by Otud6^C183A/Otud6[+]

(Villa et al., 2024)

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by Otud6^KK105705, Scer\GAL4^GMR.PY

(Villa et al., 2024)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by CG11414^HMC06381, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by Clbn^HMJ23825, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by CG11414^HMJ23120, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by Ltn1^G9156, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by Nulp1^G6757, Scer\GAL4^GMR.PU

(Park et al., 2021)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Tazelaar et al., 2020)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by eIF4B^HMS04503/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has retina phenotype, enhanceable by eIF4B^HMS04503/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has eye phenotype, enhanceable by eIF4H1^HMS04504/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has retina phenotype, enhanceable by eIF4H1^HMS04504/Scer\GAL4^GMR.PF

(Goodman et al., 2019)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has eye phenotype, non-enhanceable by Otud6^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PY

(Villa et al., 2024)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, non-enhanceable by Hsap\ATXN1^B.2Q.UAS, Scer\GAL4^GMR.PU

(Tazelaar et al., 2020)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PY, Zzzz\poly-GR^PO-36.UAS has eye phenotype, non-suppressible by Otud6^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PY

(Villa et al., 2024)

Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-36.UAS has eye phenotype, non-suppressible by Hsap\FUS^UAS.cIa, Scer\GAL4^GMR.PS

(Fujino et al., 2023)

Scer\GAL4^GMR.PS, Zzzz\poly-GR^PO-36.UAS has eye phenotype, non-suppressible by Hsap\FUS^RRMmut.UAS, Scer\GAL4^GMR.PS

(Fujino et al., 2023)

Scer\GAL4^GMR.PU, Zzzz\poly-GR^PO-36.UAS has eye phenotype, non-suppressible by Hsap\ATXN1^B.2Q.UAS, Scer\GAL4^GMR.PU

(Tazelaar et al., 2020)

Scer\GAL4^GMR.PF, Zzzz\poly-GR^PO-36.UAS has eye | adult stage phenotype, non-suppressible by SF2^GD12097, Scer\GAL4^GMR.PF

(Hautbergue et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Zzzz\poly-GR^PO-36.UAS under the control of Scer\GAL4^GMR.PU induces a eye degeneration phenotype, which is enhanced by the co-expression of CG11414^HMC06381, CG11414^HMJ23120, Clbn^HMJ23825, Nulp1^G6757, or Ltn1^G9156, leading to a severely deformed eye and necrotic patches.

(Park et al., 2021)

Xenogenetic Interactions

Statement

Reference

The severe rough eye phenotype and the severe decrease in adult locomotor activity induced by the expression of Zzzz\poly-GR^{PO-36.Scer\UAS}, under the control of Scer\GAL4^GMR.PF and Scer\GAL4^da.G32, respectively, are not suppressed by the co-expression of SF2^GD12097.

(Hautbergue et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58692

w¹¹¹⁸; P{UAS-poly-GR.PO-36}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Zzzz\poly-GR^{PO-36.Scer\UAS}

Zzzz\poly-GR^PO-36.UAS

Name Synonyms

Secondary FlyBase IDs

References (26)

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