FlyBase Allele Report: Dmel\Nav[UAS.mCherry]

General Information

Symbol

Dmel\Nav^UAS.mCherry

Species

D. melanogaster

Name

FlyBase ID

FBal0301624

Feature type

allele

Associated gene

Dmel\Nav

Associated Insertion(s)

Carried in Construct

P{UAS-Nav-mCherry}

Key Links

Transgenic product class

wild_type

(Abe et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Abe et al., 2014)

Mutagen

in vitro construct

(Abe et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-Nav-mCherry}

Cytology

Description

UAS regulatory sequences drive expression of sick tagged at the N-terminal end with mCherry.

(Abe et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nav

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body alpha-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body beta-lobe phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body alpha-lobe | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body beta-lobe | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

NOT Suppressor of

Statement

Reference

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body alpha-lobe | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Nav^UAS.mCherry, Scer\GAL4^ey-OK107 is a non-suppressor of adult mushroom body beta-lobe | somatic clone phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Abe et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} suppresses the medial axon growth defect and F-actin elevation seen in the α/β mushroom body lobes when LIMK1^{Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^ey-OK107. Dorsal axon defects are still observed.

The axon growth and increased F-actin phenotypes seen in α/β lobe mushroom body clones expressing LIMK1^{Scer\UAS.T:Ivir\HA1} are suppressed by sick^{Scer\UAS.T:Disc\RFP-mCherry}. ssh^1-63 reverses this suppression, with LIMK1^{Scer\UAS.T:Ivir\HA1} sick^{Scer\UAS.T:Disc\RFP-mCherry} ssh^1-63 triple mutant clones showing the more severe highly elevated F-actin and 'posterior arrest' (failure of axons to extend to form peduncles and lobe structures) phenotypes seen in LIMK1^{Scer\UAS.T:Ivir\HA1}, ssh^1-63 mutants. The phenotype seen in LIMK1^{Scer\UAS.T:Ivir\HA1}, ssh^1-63 double mutant clones is more severe than in either single mutant.

(Abe et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav.PU/Nav^UAS.mCherry partially rescues Nav^A384/Nav^Δ

(Abe et al., 2014)

Scer\GAL4^ey-OK107/Nav^UAS.mCherry partially rescues Nav^A384/Nav^Δ

(Abe et al., 2014)

Scer\GAL4^NP7175/Nav^UAS.mCherry partially rescues Nav^A384/Nav^Δ

(Abe et al., 2014)

Comments

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^elav.PU almost fully rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^ey-OK107 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

Expression of sick^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^NP7175 significantly rescues the axonal growth defects seen in the α/β mushroom body lobes of sick^Δ/sick^A384 mutants.

(Abe et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nav^UAS.mCherry

sick^{Scer\UAS.T:Disc\RFP-mCherry}

sick^UAS.mCherry

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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