FlyBase Allele Report: Dmel\mir-276a[KO]

General Information

Symbol

Dmel\mir-276a^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0301910

Feature type

allele

Associated gene

Dmel\mir-276a

Associated Insertion(s)

TI{TI}mir-276a^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)

Mutagen

ends-out gene targeting

(Chen et al., 2014)

FLPase

(Chen et al., 2014)

I-SceI

(Chen et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-276a^KO

Cytology

Description

The mir-276a hairpin has been deleted and replaced by a loxP cassette containing the w^+mW.hs marker.

(Chen et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm | adult stage

(Chen and Rosbash, 2016)

increased sleep

(Zhang et al., 2021)

lethal | recessive

(Chen and Rosbash, 2016, Chen et al., 2014)

partially lethal (with Df(3L)ED4457)

(Chen et al., 2014)

short lived | female | recessive

(Chen et al., 2014)

short lived | male | recessive

(Chen et al., 2014)

Phenotype Manifest In

adult heart

(Tang et al., 2024)

Detailed Description

Statement

Reference

mir-276a^KO/+ individuals show increased day-time and night-time sleep, with increased duration but decreased number of sleep episode, and decreased sleep latency; sleep recovery after sleep deprivation is significantly increased.

(Zhang et al., 2021)

mir-276a^KO homozygotes are lethal; mir-276a^KO heterozygous flies are more arrhythmic than wild-type flies (18% display arrhythmic circadian activity) and also have a significantly lower rhythmicity index. Circadian free-running period in the mutant flies that remain (at least partially) rhythmic not significantly different from wild-type controls.

(Chen and Rosbash, 2016)

Homozygous males and females show a significant decrease in median lifespan compared to controls.

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

mir-276a^KO has increased sleep phenotype, non-enhanceable by mir-276b[+]/mir-276b^KO

(Zhang et al., 2021)

Other

Statement

Reference

mir-276a^KO, mir-276b[+]/mir-276b^KO has abnormal circadian rhythm | adult stage phenotype

(Zhang et al., 2021)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mir-276a^KO, mir-276b^KO double heterozygotes show decreased circadian rhythmicity, compared to controls.

(Zhang et al., 2021)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58906

w^*; TI{TI}mir-276a^KO/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

miR-276a^KO

(Tang et al., 2024, Zhang et al., 2021)

mir-276a KO

(Chen and Rosbash, 2016)

mir-276a^KO

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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