Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4elav.PU has viable phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4Toll-6-D42 has viable phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4ey.PU has viable phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4ey.PU has visible phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4Tab2-201Y has viable phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4ppk.PG has dendrite phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4ppk.PG has dendritic branch point phenotype
Pka-C1UAS.YFP, Pka-R1UAS.CFP, Scer\GAL4ey.PU has eye phenotype
The class IV dendritic arborization neurons in flies co-expressing Pka-C1Scer\UAS.T:Avic\GFP-YFP and Pka-R1Scer\UAS.T:Avic\GFP-CFP under the control of Scer\GAL4ppk.PG show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length. The total number of dendritic branching points is also decreased.
This allele is part of a PKA-GFP sensor designed for use in FRET. Two independent subunits, Pka-C1-CFP (Pka-C1Scer\UAS.T:Avic\GFP-YFP) and Pka-R1-YFP (Pka-R1Scer\UAS.T:Avic\GFP-CFP) have been inserted into a single construct (P{UAS-Pka-R1-CFP-UAS-Pka-C1-YFP}) in order to provide equimolar expression. Each subunit is located downstream of an independent UAS promoter.