FB2026_02 , released June 18, 2026
Allele: Dmel\tbrd-2HR1
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General Information
Symbol
Dmel\tbrd-2HR1
Species
D. melanogaster
Name
FlyBase ID
FBal0302628
Feature type
allele
Associated gene
Dmel\tbrd-2
Associated Insertion(s)
TI{TI}tbrd-2HR1
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kimura and Loppin, 2015)
Mutagen
ends-out gene targeting
(Kimura and Loppin, 2015)
Progenitor genotype
Associated Insertion(s)
TI{TI}tbrd-2HR1
Cytology
Description

The entire tbrd-2 gene has been replaced by a loxP cassette containing a w+mW.hs marker.

(Kimura and Loppin, 2015)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:19,259,787..19,262,388 [+]
Comment:

Ends-out homologous recombination has resulted in the entire tbrd-2 gene being replaced by a w marker. (extent of deletion inferred from primer sequences).

(Kimura and Loppin, 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      tbrd-2HR mutant spermatids are produced, but contain nuclei of various sizes and irregular mitochondria, and display aberrant cytokinesis at the onion stage. Primary spermatocyte morphology at earlier stages is also unaffected.

      No chromosome defects are observed in G2 phase chromosomes of tbrd-2HR mutant testes. However at prophase I when chromatid starts to condense into the typical three main chromosome masses, the two prominent asters of microtubules present in wild type spermatocytes are either not formed , aberrant in aspect or present in excessive numbers. In the subsequent metaphase I. anaphase I and telophase I sister chromatid segregation appears abnormal.

      (Kimura and Loppin, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      tbrd-2HR1 has spermatid phenotype, non-enhanceable by tbrd-12

      (Kimura and Loppin, 2015)
      NOT Enhancer of
      Statement
      Reference

      tbrd-2HR1 is a non-enhancer of spermatid phenotype of tbrd-12

      (Kimura and Loppin, 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The spermatid phenotype observed in tbrd-12 tbrd-2HR double mutants is similar to that seen in either mutant alone. Primary spermatocyte morphology at earlier stages is also unaffected.

      (Kimura and Loppin, 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      tbrd-2HR1 is rescued by tbrd-2EGFP,Tag:polyHis

      (Kimura and Loppin, 2015)
      Comments

      Expression of tbrd-2T:Avic\GFP-EGFP,T:Zzzz\His6 rescues the sterility and spermatid defects seen in tbrd-2HR mutant males.

      (Kimura and Loppin, 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      tbrd-2HR1
      (Kimura and Loppin, 2015)
      tbrd-2HR
      (Kimura and Loppin, 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)