FlyBase Allele Report: Dmel\azot[KO]

The azot coding region has been deleted by homologous recombination and replaced with an attP site and P1\loxP-flanked w marker. The boundaries of the deleted region were inferred from primer sequences.

(Merino and Moreno, 2015.4.11)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death

(Merino et al., 2015)

radiation sensitive

(Merino et al., 2015)

short lived

(Merino et al., 2015)

viable

(Merino et al., 2015)

visible

(Merino et al., 2015)

Phenotype Manifest In

wing

(Merino et al., 2015)

Detailed Description

Statement

Reference

These mutants are short lived compared to controls.

(Merino et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

azot^KO/azot^KO is an enhancer of abnormal neuroanatomy | adult stage | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

azot^KO/azot^KO is an enhancer of abnormal memory | adult stage | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Suppressor of

Statement

Reference

azot^KO/azot^KO is a suppressor of increased cell death | adult stage | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Phenotype Manifest In

Enhancer of

Statement

Reference

azot^KO/azot^KO is an enhancer of adult brain | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Suppressor of

Statement

Reference

azot^KO/azot^KO is a suppressor of adult brain | RU486 conditional phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^{elav.Switch.PO}

(Coelho et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The loss of 'loser' cell elimination seen in azot^KO mutant cells following dm-induced cell competition is rescued upon expression of azot^T:Disc\RFP.

Expression of two copies of azot^T:Disc\RFP rescues the wing defects seen in homozygous azot^KO mutant flies.

Expression of two copies of azot^T:Disc\RFP rescues the increase in vacuoles seen in azot^KO mutant brains.

Expression of two copies of azot^T:Disc\RFP rescues the reduced lifespan seen in azot^KO mutant brains.

(Merino et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

azot^KO

(Cachoux et al., 2023, Merino and Moreno, 2015.4.11)

azot{KO; w+}

(Coelho et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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