FlyBase Allele Report: Dmel\miple1[UAS.Tag:HA]

General Information

Symbol

Dmel\miple1^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0302845

Feature type

allele

Associated gene

Dmel\miple1

Associated Insertion(s)

Carried in Construct

P{UAS-miple1-HA}

Also Known As

UAS-miple

Key Links

Transgenic product class

wild_type

(Toledano-Katchalski et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Toledano-Katchalski et al., 2007)

Mutagen

in vitro construct

(Muñoz-Soriano et al., 2013, Toledano-Katchalski et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-miple1-HA}

Cytology

Description

UASt regulatory sequences drive expression of miple1 coding sequences fused to a Tag:HA tag at the C-terminal.

(Toledano-Katchalski et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

miple1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of miple1^{Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^hs.2sev or Scer\GAL4^GMR.PU generates an ommatidial rotation eye development phenotype.

Expression of miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU results in a clear rough eye phenotype, in which tangential sections display rotation defects but mainly defects in photoreceptor recruitment. This phenotype is dosage-sensitive, as it is markedly suppressed by reducing miple1 expression through expression of miple1^GD4826.

(Muñoz-Soriano et al., 2013)

Expression of miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^twi.PG results in a loss of pericardial and cardiac cells in 1-2 cells in embryos at later developmental stages. The somatic musculature is also affected, with a lack of muscles in 1-2 segments, especially at the dorsal aspects of the embryo.

(Toledano-Katchalski et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, enhanceable by Ptp99A¹

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has ommatidium phenotype, enhanceable by Ptp99A¹

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, enhanceable by nmo^P1

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has ommatidium phenotype, enhanceable by nmo^P1

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, enhanceable by nmo^P1

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has ommatidium phenotype, enhanceable by nmo^P1

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has ommatidium phenotype, enhanceable by nmo^UAS.cUa/Scer\GAL4^hs.2sev

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, enhanceable by nmo^DB

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has ommatidium phenotype, enhanceable by nmo^DB

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, enhanceable by aos^Δ7

(Muñoz-Soriano et al., 2013)

NOT Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, non-enhanceable by aos^Δ7

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has ommatidium phenotype, non-enhanceable by aos^Δ7

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, non-enhanceable by nmo^UAS.cUa/Scer\GAL4^hs.2sev

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has ommatidium phenotype, non-enhanceable by nmo^UAS.cUa/Scer\GAL4^hs.2sev

(Muñoz-Soriano et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, suppressible by Ptp99A¹

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, suppressible by arm⁴/arm[+]

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, suppressible by shg[+]/shg²

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, suppressible by Egfr^DN.UAS/Scer\GAL4^hs.2sev

(Muñoz-Soriano et al., 2013)

Scer\GAL4^GMR.PU, miple1^UAS.Tag:HA has eye phenotype, suppressible by Egfr^f24

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, suppressible by arm⁴/arm[+]

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, suppressible by shg[+]/shg²

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, suppressible by Egfr^DN.UAS, Scer\GAL4^hs.2sev

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev, miple1^UAS.Tag:HA has eye phenotype, suppressible by Egfr^f24

(Muñoz-Soriano et al., 2013)

NOT Enhancer of

Statement

Reference

Scer\GAL4^hs.2sev/miple1^UAS.Tag:HA is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Muñoz-Soriano et al., 2013)

Scer\GAL4^hs.2sev/miple1^UAS.Tag:HA is a non-enhancer of eye phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Muñoz-Soriano et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

A nmo^P1 mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

Dosage reduction of nmo, through a nmo^P1 mutant background is able to enhance the eye roughness seen in Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} mutants.

Expression of miple1^{Scer\UAS.T:Ivir\HA1} does not enhance the ommatidial rotation eye phenotype found upon expression of nmo^Scer\UAS.cUa under the control of Scer\GAL4^hs.2sev.

Dosage reduction of nmo, through a nmo^P1 or nmo^DB mutant background is able to enhance the eye roughness seen in Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} mutants.

A Ptp99A¹ mutant background suppresses the eye roughness seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU.

A Ptp99A¹ mutant background significantly enhances the ommatidial rotation phenotype found in Scer\GAL4^hs.2sev>miple1^{Scer\UAS.T:Ivir\HA1} mutants.

A Ptp99A¹ mutant background significantly enhances the ommatidial rotation phenotype found in Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} mutants.

An arm⁴ heterozygous mutant background suppresses the eye roughness seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU.

A shg² heterozygous mutant background suppresses the eye roughness seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU.

Downregulation of Egfr, through expression of Egfr^DN.Scer\UAS or a Egfr^f24 mutant background, suppresses the Scer\GAL4^GMR.PU>miple1^{Scer\UAS.T:Ivir\HA1} eye phenotype.

An aos^Δ7 mutant background enhances the eye roughness seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PU.

A nmo^P1 or nmo^DB mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

A Ptp99A¹ mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

A shg² heterozygous mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

An arm⁴ heterozygous mutant background enhances the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

Downregulation of Egfr, through expression of Egfr^DN.Scer\UAS or a Egfr^f24 mutant background, does not alter the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

An aos^Δ7 mutant background does not alter the ommatidial rotation phenotype seen in flies expressing miple1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.2sev.

(Muñoz-Soriano et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

miple1^UAS.Tag:HA is rescued by miple1^JF02904/Scer\GAL4^GMR.PU

(Muñoz-Soriano et al., 2013)

Comments

(Muñoz-Soriano et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

miple1^{Scer\UAS.T:Ivir\HA1}

miple1^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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