FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\sflRNAi.UAS.THFC
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General Information
Symbol
Dmel\sflRNAi.UAS.THFC
Species
D. melanogaster
Name
FlyBase ID
FBal0302957
Feature type
allele
Associated gene
Dmel\sfl
Associated Insertion(s)
Carried in Construct
P{UAS-sfl.RNAi.THFC}
Key Links
Transgenic product class
RNAi_reagent
(Yu et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Yu et al., 2015)
Mutagen
in vitro construct
(Yu et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-sfl.RNAi.THFC}
Cytology
Description

UAS regulatory sequences drive expression of an RNAi construct targeting sfl.

(Yu et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
sfl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  azoospermia
      CEA
      (Yu et al., 2015)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of sfldsRNA.Scer\UAS.THFC in somatic cells during gonadal development by Scer\GAL4esg-NP5130 results in oblong-shaped testes. Approximately 97% of the Scer\GAL4esg-NP5130>sfldsRNA.Scer\UAS.THFC males are sterile. Approximately half of the mutant testes lose the normal tubular shape and show morphological defects. Germ cells at each spermatogenic stage are detected in the squashed testes with morphological defects of most of the mutant males. The morphological defects include clusters of elongated spermatid nuclei. The clusters of elongated nuclei are also seen in morphologically normal testes of the mutants.

      (Yu et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sflRNAi.UAS.THFC
      sfldsRNA.Scer\UAS.THFC
      sfldsRNA.UAS.THFC
      Name Synonyms
      Secondary FlyBase IDs
        References (2)