FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\S6KL140
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General Information
Symbol
Dmel\S6KL140
Species
D. melanogaster
Name
FlyBase ID
FBal0302974
Feature type
allele
Associated gene
Dmel\S6KL
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Zhao et al., 2015)
Mutagen
P-element activity
(Zhao et al., 2015)
Progenitor genotype
P{EPgy2}S6KLEY10051
(Zhao et al., 2015)
Cytology
Description

Imprecise excision of P{EPgy2}S6KLEY10051 has resulted in a 2553bp deletion that removes exons 1-5 of S6KL, which includes part of the kinase domain.

(Zhao et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The neuromuscular junction synapses of homozygous S6KL140 mutants are obviously overgrown with extra boutons. The mean satellite bouton number is four fold higher than in controls. A similar phenotype is seen in S6KL140/Df(1)ED7413.

      S6KL140 mutants exhibit ultrastructural defects in the NMJ: the subsynaptic reticulum appears largely normal, but the synaptic vesicle (SV) density within the whole presynaptic bouton is moderately but significantly reduced compared to wild type. A small subpopulation of vesicles at the active zones is larger in S6KL140 mutants compared to controls.

      The mean excitatory junction potential (EJP) amplitude is not significantly altered in S6KL140 mutants compared to controls, but the miniature EJP (quantal content) is higher.

      S6KL140 mutant larvae exhibit reduced synaptic vesicle endocytosis: the level of membrane uptake (dye internalisation) in mutant synaptic boutons in response to K[+] stimulation is significantly reduced compared to controls.

      In contrast to wild type, EJP amplitudes in S6KL140 mutant larvae fall significantly during high frequency stimulation.

      (Zhao et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      S6KL140 has embryonic/larval neuromuscular junction phenotype, suppressible by tkv7/tkv[+]

      (Zhao et al., 2015)

      S6KL140 has NMJ bouton | increased number phenotype, suppressible by tkv7/tkv[+]

      (Zhao et al., 2015)

      S6KL140 has embryonic/larval neuromuscular junction phenotype, suppressible by Madk00237/Mad[+]

      (Zhao et al., 2015)

      S6KL140 has NMJ bouton | increased number phenotype, suppressible by Madk00237/Mad[+]

      (Zhao et al., 2015)

      S6KL140 has embryonic/larval neuromuscular junction phenotype, suppressible by witA12/wit[+]

      (Zhao et al., 2015)

      S6KL140 has NMJ bouton | increased number phenotype, suppressible by witA12/wit[+]

      (Zhao et al., 2015)
      Other
      Statement
      Reference

      Dadj1E4/Dad[+], S6KL140 has NMJ bouton | increased number phenotype

      (Zhao et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of tkv7 completely suppresses the increase in total bouton number seen in S6KL140 mutants.

      One copy of Madk00237 suppresses the increase in total bouton number seen in S6KL140 mutants.

      One copy of witA12 suppresses the increase in total bouton number seen in S6KL140 mutants.

      S6KL140/+ ; Dadj1E4/+ transheterozygous mutants exhibit an increase in total bouton number not seen in either heterozygote alone.

      (Zhao et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      S6KL140 is rescued by Scer\GAL4elav.PU/S6KLUAS.cZa

      (Zhao et al., 2015)
      Not rescued by

      S6KL140 is not rescued by Scer\GAL4Mhc.PU/S6KLUAS.cZa

      (Zhao et al., 2015)

      S6KL140 is not rescued by Scer\GAL4elav.PU/S6KLUAS.K193Q

      (Zhao et al., 2015)
      Comments

      Pre-synaptic expression of S6KLScer\UAS.cZa under the control of Scer\GAL4elav.PU fully rescues the neuromuscular junction overgrowth phenotype seen in homozygous S6KL140 mutants. The increase in quantal content seen in S6KL140 mutant flies is also rescued.

      Post-synaptic expression of S6KLScer\UAS.cZa under the control of Scer\GAL4Mhc.PU does not rescue the neuromuscular junction overgrowth phenotype seen in homozygous S6KL140 mutants.

      Expression of S6KLScer\UAS.K193Q under the control of Scer\GAL4elav.PU does not rescue the neuromuscular junction overgrowth phenotype seen in homozygous S6KL140 mutants.

      (Zhao et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      S6KL140
      (Zhao et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)