FlyBase Allele Report: Dmel\Hr39[UAS.cBa]

General Information

Symbol

Dmel\Hr39^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Boulanger

FlyBase ID

FBal0304304

Feature type

allele

Associated gene

Dmel\Hr39

Associated Insertion(s)

Carried in Construct

P{UAS-Hr39.B}

Also Known As

UAS-Hr39

Key Links

Transgenic product class

cDNA

(Boulanger et al., 2011)

wild_type

(Boulanger et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Boulanger et al., 2011)

wild_type

(Boulanger et al., 2011)

Mutagen

in vitro construct

(Boulanger et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Hr39.B}

Cytology

Description

A full-length 3.3kb Hr39 cDNA fragment is cloned downstream of UAS regulatory sequences.

(Boulanger et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hr39

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Phenotype Manifest In

gamma Kenyon cell, with Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Detailed Description

Statement

Reference

Ectopic expression of Hr39^Scer\UAS.cBa driven by Scer\GAL4^Tab2-201Y in developing γ neurons blocks pruning. Although some γ neurons seem pruned normally, 100% of the mushroom bodies predominantly display γ neurons that retain their larval characteristics. In mutant neuroblast clones, there is a mixture of unpruned and apparently pruned &gr; neurons. Similarly, in two-cell mutant clones, two mutant neurons are clearly unpruned whereas two others appear normal. Only γ neurons appear affected by Hr39^Scer\UAS.cBa ectopic expression in adult mushroom body neurons.

(Boulanger et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y has abnormal neuroanatomy phenotype, enhanceable by ftz-f1^ex7

(Boulanger et al., 2011)

Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y has abnormal neuroanatomy phenotype, enhanceable by EcR^B1.UAS, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Suppressed by

Statement

Reference

Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y has abnormal neuroanatomy phenotype, suppressible | partially by ftz-f1^β.UAS.cBa, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y has gamma Kenyon cell phenotype, enhanceable by ftz-f1^ex7

(Boulanger et al., 2011)

Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y has gamma Kenyon cell phenotype, enhanceable by EcR^B1.UAS, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Suppressed by

Statement

Reference

Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y has gamma Kenyon cell phenotype, suppressible | partially by ftz-f1^β.UAS.cBa, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ftz-f1^{β.Scer\UAS.cBa} in γ-neurons partially rescues the dendritic pruning found upon expression of Hr39^Scer\UAS.cBa (with both transgenes under the control of Scer\GAL4^Tab2-201Y).

A ftz-f1^ex7 heterozygous background enhances the mutant pruning phenotype seen in flies expressing Hr39^Scer\UAS.cBa in γ neurons (with both transgenes under the control of Scer\GAL4^Tab2-201Y).

Expression of EcR^B1.Scer\UAS in Hr39^Scer\UAS.cBa neuroblast clones (under the control of Scer\GAL4^Tab2-201Y) restores wild-type γ neuron remodelling.

(Boulanger et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

64283