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General Information
Symbol
Dmel\ftz-f1ex7
Species
D. melanogaster
Name
FlyBase ID
FBal0120497
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The entire exon coding for the zinc finger DBD region is deleted.

Excision of the P{lacW} element resulting in a deletion with the 3' endpoint in the first intron.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In ftz-f1ex7/ftz-f103649 mutants, no pupation occurs; when ftz-f1hs.PM is induced at 8hr APF or later, pupation occurs and is delayed (length of delay depends on the timing of the heat shock).

Nervous system MARCM somatic cell clones of ftz-f1ex7 mutants, under the control of Scer\GAL4Tab2-201Y retain their larval-type branching of axons into the adult stage. The mutant phenotype can also be seen in single- and two-cell clones. Approximately 80% of ftz-f1ex7 mutant cell clones appear wild-type, with 20% appearing unpruned. ftz-f1ex7 neuroblast clones are indistinguishable from wild-type at the third instar larval stage but display no signs of pruning at 24 hours after puparium formation, in contrast to their wild-type counterparts in which most axons are fully pruned.

ftz-f1ex7 heterozygotes display normal γ neuron pruning.

ftz-f1ex7 homozygotes which have been rescued by expression of ftz-f1hs.PM using heat shock 13-16 hours after egg laying (AEL) survive for a few days, but do not progress to second instar larvae even 24 hours after hatching. If the larvae are heat shocked again at 33oC for 60 minutes during the later period of the first instar stage (within 42-47 hours AEL) about 30% succeed in ecdysis and progress to the second instar stage. Again, they survive for a few days, but cannot progress to third instar larvae. The larvae have two pairs of mouth hooks, cephalopharyngeal skeletons, anterior and posterior spiracles and double tubular tracheae, and cuticle for the third instar is present in addition to that for the second instar. This indicates that the larvae have prepared the structures appropriate to third instar larvae, but failed in the second ecdysis. The rescued second instar larvae progress to third instar larvae upon heat shock at 33oC for 60 minutes during the late period of the second instar stage (within 66-71 hours AEL), although efficiency of rescue is very low (only 2.5% progress to prepupae). Most ftz-f1ex7/ftz-f103649 transheterozygotes die before reaching the prepupal stage, but 3% do progress to prepupae. The development of this 3% arrests before prepupal stage P4. Most ftz-f1ex7/ftz-f103649 transheterozygotes die before reaching the prepupal stage, but 3% do progress to prepupae. The development of this 3% arrests before prepupal stage P4. Larval salivary glands are still present even 24 hours after puparium formation (in contrast to wild type). The optic lobes do not expand as in wild type and the subesophageal neuromeres and thoracic neuromeres do not separate. Arrested development is also manifested as leg elongation, edge constriction of the wing disc and morphological changes in the midgut and Malpighian tubules.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

A ftz-f1ex7 heterozygous background enhances the mutant pruning phenotype seen in flies expressing Hr39Scer\UAS.cBa in γ neurons (with both transgenes under the control of Scer\GAL4Tab2-201Y).

Expression of EcRB1.Scer\UAS (under the control of Scer\GAL4Tab2-201Y) in ftz-f1ex7 neuroblast clones restores normal γ neuron remodelling.

When ftzhs.PSb and ftz-f1α.hs are coexpressed in ftz-f1ex7, the 'anti-ftz' cuticle phenotype is suppressed, but if ftz-f1ΔAF2C.hs is coexpressed with ftzhs.PSb, the phenotype is not suppressed.

Salivary gland histolysis is rescued in most ftz-f1ex7/ftz-f103649 transheterozygotes when brBRcore.TNT.Q1.Z1.hs is expressed using heat shock at 10 hours after puparium formation, although other ftz-f1 mutant phenotypes are not rescued in these animals. Salivary gland histolysis is rescued in about half of ftz-f1ex7/ftz-f103649 transheterozygotes when brZ2.hs is expressed using heat shock at 10 hours after puparium formation. Salivary gland histolysis is not rescued in ftz-f1ex7/ftz-f103649 transheterozygotes when either brBRcore.NS.Z3.hs or brBRcore.Z4.hs is expressed using heat shock at 10 hours after puparium formation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

In ftz-f1ex7/ftz-f103649 mutants, no pupation occurs when ftz-f1hs.PM is induced at 4 or 5hr APF, but 75 or 100% of animals become pupa at 12.3 or 12.6 APF on average when ftz-f1hs.PM is induced at 6 or 7hr APF, respectively.

Expression of ftz-f1β.Scer\UAS.cBa in MARCM ftz-f1ex7 mutant cell clones under the control of Scer\GAL4Tab2-201Y fully rescues the mutant phenotype.

About 40% of ftz-f1ex7 homozygous embryos hatch when ftz-f1hs.PM is expressed using heat shock at 13-16 hours after egg laying. ftz-f1ex7 lethality is not rescued by heat shock at other times. ftz-f1hs.PM rescues ftz-f1ex7/ftz-f103649 animals that are arrested as prepupae before stage P4; the rescued animals are able to become pupae and a fraction of them develop until at least stage P12. Efficient rescue is seen when the animals are heat shocked 6-10 hours after puparium formation.

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Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)