FlyBase Allele Report: Dmel\br[BRcore.Z4.hs]

General Information

Symbol

Dmel\br^BRcore.Z4.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0057738

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

P{hs-BRC-Z4}

Also Known As

hs-BR-Z4

Key Links

Transgenic product class

cDNA

(Crossgrove et al., 1996)

wild_type

(Crossgrove et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Crossgrove et al., 1996)

wild_type

(Crossgrove et al., 1996)

Mutagen

in vitro construct

(Bayer et al., 1997, Sandstrom et al., 1997, Crossgrove et al., 1996)

Progenitor genotype

Carried in construct

P{hs-BRC-Z4}

Cytology

Description

br cDNA 28-I is expressed from a heat inducible Hsp70 promoter.

(Crossgrove et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | heat sensitive

(Zhou and Riddiford, 2002)

lethal | heat sensitive

(Bayer et al., 1997)

lethal - all die before end of larval stage | heat sensitive

(Zhou et al., 2004)

some die during larval stage | heat sensitive

(Zhou et al., 2004)

Phenotype Manifest In

chaeta | heat sensitive

(Zhou and Riddiford, 2002)

dorsal appendage | ectopic | cold sensitive

(Tzolovsky et al., 1999)

tormogen cell | heat sensitive

(Zhou and Riddiford, 2002)

Detailed Description

Statement

Reference

Over-expression of four copies of br^BRcore.Z4.hs using heat shock at 5 hours after ecdysis to the second instar causes a high rate of lethality at approximately 26 hours after the heat shock (the time of ecdysis from second to third instar). Double mouthhooks are seen in these dead larvae.

(Zhou et al., 2004)

Animals carrying four copies of br^BRcore.Z4.hs and heat shocked at 56 hours after puparium formation have essentially full length bristles that are unpigmented. The sockets of the macrochaetae are also not tanned.

(Zhou and Riddiford, 2002)

In response to heatshock, ectopic dorsal appendage material is produced in the dorsal anterior region of the eggshell. 0-3 hours post heatshock about 5% of the eggs produced are abnormal, and 95% of eggs laid hatch. 3-5 post heatshock 43% of the eggs produced are abnormal, and only 4% of eggs laid hatch. From 5-24 hours post heatshock about 3/4 of eggs produced are abnormal, and 15% of eggs laid hatch. 24-48 hours post heatshock, about a 1/5 of eggs produced are abnormal, and 97% of eggs laid hatch. Viability is recovered to about 95% in 2 days after heatshock.

(Tzolovsky et al., 1999)

A 37^oC heat shock causing expression of br^BRcore.Z4.hs during the third larval instar or early prepupal stage results in lethality within a few hours of the heat shock. Heat shock induced expression of br^BRcore.Z4.hs during late prepupal or pupal stages has no effect.

(Bayer et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

br^BRcore.Z4.hs is a non-suppressor of embryonic/larval salivary gland phenotype of ftz-f1⁰³⁶⁴⁹/ftz-f1^ex7

(Yamada et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Salivary gland histolysis is not rescued in ftz-f1^ex7/ftz-f1⁰³⁶⁴⁹ transheterozygotes when br^BRcore.Z4.hs is expressed using heat shock at 10 hours after puparium formation.

(Yamada et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

br^BRcore.Z4.hs partially rescues br^rbp-1

(Bayer et al., 2003)

br^BRcore.Z4.hs partially rescues br^rbp-5

(Bayer et al., 1997)

br^BRcore.Z4.hs partially rescues br^2Bc-2

(Bayer et al., 1997)

br^BRcore.Z4.hs partially rescues br^rbp-1

(Bayer et al., 1997)

br^BRcore.Z4.hs partially rescues br^rbp-1

(Sandstrom et al., 1997)

Fails to rescue

br^BRcore.Z4.hs fails to rescue br²⁸

(Bayer et al., 1997)

br^BRcore.Z4.hs fails to rescue br⁵

(Bayer et al., 1997)

br^BRcore.Z4.hs fails to rescue br^npr-3

(Bayer et al., 1997)

br^BRcore.Z4.hs fails to rescue br^npr-7

(Bayer et al., 1997)

Comments

Expression of one copy of br^BRcore.Z4.hs in br^rbp-1 mutant flies results rescue of only 7% of animals to eclosion. This increases to 13% when two copies are used. Mutant br^rbp-1 flies rescued with br^BRcore.Z4.hs typically die within 1-2 days of eclosion. These animals have a protracted proboscis and are most likely unable to feed. The malformed leg phenotype found in br^rbp-1 animals that develop to late pupal stages is rescued in approximately 50% of cases by the expression of two br^BRcore.Z4.hs transgenes.

(Bayer et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

36575

w¹¹¹⁸; P{hs-BRC-Z4}11

Notes on Origin

Discoverer

Comments

The br Z4 protein isoform encoded by br^BRcore.Z4.hs provides partial rbp⁺ function.

(Bayer et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

br^BRcore.Z4.hs

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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