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General Information
Symbol
Dmel\brnpr-3
Species
D. melanogaster
Name
FlyBase ID
FBal0013157
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
npr13, l(1)npr-1, npr3, npr-1, brnpr3
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

100% of brrbp-2/brnpr-3 larvae are able to pupate, but ~15% die as early pupae. 80% of these survive to late pharate adult stage, with ~20% failing to eclose. brnpr-3/+ flies show the same susceptibility to methoprene as wild-type flies.

brnpr-3/+ flies show a wild-type number of DLM fibers and normal TDT patterning in the adult thorax.

The wing marginal sensory neurons fail to differentiate in brnpr-3 mutant clones and brnpr-3 Minute mutant clones. The loss of the marginal neurons is permanent as chemosensory bristles are never observed in adult wings with brnpr-3 clones encompassing the wing margin.

brnpr-3 mutants exhibit a significant reduction in encapsulation response. Lymph glands of older, developmentally arrested mutant larvae are of normal size and do not show hypertrophy.

brnpr-3/Y eye discs show ommatidial disorganisation and signs of furrow failure, including mature ommatidial clusters at the furrow. Homozygous clones in the eye disc show defects in ommatidial organisation, including the wrong number of photoreceptors in clusters. Excess numbers of R8 cells in a cluster are seen. Homozygous clones spanning the morphogenetic furrow are not associated with any retardation of the furrow. Clones at the posterior margin of the disc do not show any visible defects in furrow initiation. Homozygous clones in the adult eye result in a scar.

Homozygotes arrest development at the end of the larval period, do not pupariate and die after several days as wandering third instar larvae.

Lethality acts in the late third instar.

The Pig1 to Sgs4 switch fails to occur during mid-third instar.

Lethality acts at the late third instar stage.

Failure of salivary gland degeneration. Reduction in dorso-ventral class of indirect flight muscles.

18--28% penetrance of zip interaction with brnpr-3 heterozygous females.

Wandering third instar larvae initially show normal CNS histology, later widespread loss of tissue integrity is seen.

Imaginal discs of homozygous larvae are normal.

Imaginal discs form swollen vesicles.

No maternal effect.

Larvae do not pupate, but can survive for weeks after reaching the size of the mature wild-type larva. Larval imaginal discs are normal on the 4th day after hatching, however they become bloated and a highly distorted by the 8th-10th day of larval life. The ring gland is normal in size on the 8th-10th day of larval life.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference

brnpr-3/br[+] is an enhancer of visible | dominant phenotype of B1

Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference

brnpr-3/br[+] is an enhancer of eye phenotype of B1

Other
Additional Comments
Genetic Interactions
Statement
Reference

The lethality is enhanced in brrbp-2/brnpr-3 Rst(1)JH3/Rst(1)JH3 double mutants compared to brrbp-2/brnpr-3 mutants, with no flies surviving beyond the pupal stage.

There is a significant reduction in the number of DLM fibers in brnpr-3/+; twi1/+ double mutants (5.8 fibers vs 6 in wild type) and an even greater reduction in brnpr-3/+; twi1/+, Mef2P544/+ triple mutants (5.2 fibers). However, no such reduction is seen in brnpr-3/+; Mef2P544/+ double mutants.

brnpr-3/+; Mef2P544/+ double heterozygotes display higher levels of defects in TDT organization than either single heterozygote, including the presence of ectopic fibers in the lumen. 82% of brnpr-3/+; twi1/+, Mef2P544/+ triple mutants show defects in TDT patterning (a higher level than any of the double mutant combinations), including additional small cells, internal fibers and the rerouting of some fibers to the periphery.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

brnpr-3 is partially rescued by brZ2.hs

Comments

Lethality is not rescued by brBRcore.Q1.Z1.hs, brBRcore.TNT.Q1.Z1.hs, brZ2.hs, brBRcore.NS.Z3.hs or brBRcore.Z4.hs when expressed using heat shocks of either 33oC or 37oC. brnpr-3 third instar larvae expressing brZ2.hs show further development than control brnpr-3 larvae, forming normal looking puparia with everted spiracles, a tanned cuticle and imaginal discs that have initiated morphogenesis.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer

Kiss.

Comments
Comments

Analysis of Ecol\lacZSgs3.GLX3.3 expression in salivary glands mosaic for brnpr-3 suggests a cell-autonomous requirement for br+ function for the expression of Sgs3 in the salivary gland.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (14)
References (48)