100% of brrbp-2/brnpr-3 larvae are able to pupate, but ~15% die as early pupae. 80% of these survive to late pharate adult stage, with ~20% failing to eclose. brnpr-3/+ flies show the same susceptibility to methoprene as wild-type flies.
brnpr-3/+ flies show a wild-type number of DLM fibers and normal TDT patterning in the adult thorax.
The wing marginal sensory neurons fail to differentiate in brnpr-3 mutant clones and brnpr-3 Minute mutant clones. The loss of the marginal neurons is permanent as chemosensory bristles are never observed in adult wings with brnpr-3 clones encompassing the wing margin.
brnpr-3 mutants exhibit a significant reduction in encapsulation response. Lymph glands of older, developmentally arrested mutant larvae are of normal size and do not show hypertrophy.
brnpr-3/Y eye discs show ommatidial disorganisation and signs of furrow failure, including mature ommatidial clusters at the furrow. Homozygous clones in the eye disc show defects in ommatidial organisation, including the wrong number of photoreceptors in clusters. Excess numbers of R8 cells in a cluster are seen. Homozygous clones spanning the morphogenetic furrow are not associated with any retardation of the furrow. Clones at the posterior margin of the disc do not show any visible defects in furrow initiation. Homozygous clones in the adult eye result in a scar.
Homozygotes arrest development at the end of the larval period, do not pupariate and die after several days as wandering third instar larvae.
Lethality acts in the late third instar.
The Pig1 to Sgs4 switch fails to occur during mid-third instar.
Lethality acts at the late third instar stage.
Failure of salivary gland degeneration. Reduction in dorso-ventral class of indirect flight muscles.
18--28% penetrance of zip interaction with brnpr-3 heterozygous females.
Wandering third instar larvae initially show normal CNS histology, later widespread loss of tissue integrity is seen.
Imaginal discs of homozygous larvae are normal.
Imaginal discs form swollen vesicles.
Larvae do not pupate, but can survive for weeks after reaching the size of the mature wild-type larva. Larval imaginal discs are normal on the 4th day after hatching, however they become bloated and a highly distorted by the 8th-10th day of larval life. The ring gland is normal in size on the 8th-10th day of larval life.