Open Close
General Information
Symbol
Dmel\brZ2.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0057737
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

br cDNA cD5 is expressed from a heat inducible Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ubiquitous expression of brZ2.hs does not cause malrotation of male genitalia. Partial rescue of br5/Y mutants, achieved by driving expression of the brZ2.hs transgene by three heat shocks at 37oC starting in the third instar results in small numbers of pharate adults, 93% of which have malrotated genitalia.

Over-expression of four copies of brZ2.hs using heat shock at 5 hours after ecdysis to the second instar causes a high rate of lethality at approximately 26 hours after the heat shock (the time of ecdysis from second to third instar). Double mouthhooks are seen in these dead larvae.

Animals carrying four copies of brZ2.hs and heat shocked at 56 hours after puparium formation develop into morphologically normal pharate adults.

In response to heatshock, ectopic dorsal appendage material is produced in the dorsal anterior region of the eggshell. 3-5 post heatshock about half of the eggs produced are abnormal, 5% of eggs laid hatching. From 5-24 hours post heatshock about 3/4 of eggs produced are abnormal, and 12% of eggs laid hatch. 24-48 hours post heatshock, about a 1/3 of eggs produced are abnormal, and 93% of eggs laid hatch.

A 37oC heat shock causing expression of brZ2.hs during the pupal stage results in lethality. Heat shock induced expression of brZ2.hs during larval or prepupal stages has no effect.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Salivary gland histolysis is rescued in about half of ftz-f1ex7/ftz-f103649 transheterozygotes when brZ2.hs is expressed using heat shock at 10 hours after puparium formation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues

brZ2.hs partially rescues br5

brZ2.hs partially rescues br28

Fails to rescue
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

The br Z2 protein isoform encoded by brZ2.hs provides br+ function and partial 2Bc+ function.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
brZ2.hs
Name Synonyms
Secondary FlyBase IDs
    References (9)