The puffing pattern of salivary glands in mutant larvae corresponds to the normal developmental sequence, with all puff stages from PS1 to PS10-11 being present. Mutants die at the white prepupa stage.
Homozygotes arrest development in 0-hour prepupae with an untanned larval cuticle and imaginal discs are not everted. 17% of br28/Y males carrying two copies of brZ2.hs are rescued to eclosion when brZ2.hs is expressed using 37oC heat shocks twice during the third larval instar stage and once during pupariation. br28/Y males carrying two copies of brZ2.hs can advance to prepupal or pupal stages when brZ2.hs is expressed using 33oC heat shocks. brBRcore.Q1.Z1.hs, brBRcore.TNT.Q1.Z1.hs, brBRcore.NS.Z3.hs and brBRcore.Z4.hs does not rescue the lethality of br28/Y males when expressed using heat shocks of either 33oC or 37oC.
The Pig1 to Sgs4 switch fails to occur during mid-third instar.