br23RL retains the P{lacW}br2Bab-8 insertion present in the br2Bab-8 progenitor allele and also contains a second insertion (P{lacW}br23RL), inserted 3531bp downstream of the original insertion, in an intron.
Lethality occurs during the third larval instar. The early ecdysone puffs 50CD, 74E, 75B and the late puffs 29F, 46F, 62E, 71CE, 78C, 82F, 85D, 93D and 98E are not present in mutant larvae. The intermolt puffs 34A, 25AB and 68C do not disappear in these mutants.