Ubiquitous expression of brBRcore.NS.Z3.hs does not cause malrotation of male genitalia.
83% of larvae expressing four copies of brZ3.Scer\UAS induced by heat shock at 5 hours after ecdysis to the second instar fail to initiate the third larval moult but continue to eat and grow, far exceeding the size of a normal second instar larva. These larvae eventually pupariate approximately 93 hours after the heat-shock treatment, forming "L2 prepupae" which are characterised by their uneverted, single club-shaped anterior spiracles and small mouthhooks with a few teeth (characteristic of wild-type second instar larvae). Approximately 97% of the L2 prepupae successfully evert their imaginal discs and head, and most of them develop up to stage P8, showing pale yellow pigmentation in the eyes. In a very few cases, they become pharate adults.
Expression of brBRcore.NS.Z3.hs in second instar larvae induced by heat shock at least 7 hours after ecdysis to the second instar does not prevent the animals forming a pharate third instar, although most of the animals do not ecdyse.
Expression of brBRcore.NS.Z3.hs in early first instar larvae by heat shock at 2-5 hours after hatching results in larvae that ecdyse to the second instar, but with an 18 hour delay at 25[o]C.
Expression of brBRcore.NS.Z3.hs in first instar larvae by heat shock at 4 hours after hatching followed by two heat shocks at approximately 16 hours and 28 hours prevents 96% of the larvae from moulting to the second instar.
Animals carrying four copies of brBRcore.NS.Z3.hs and heat shocked at 48 hours after puparium formation (APF) develop into morphologically normal pharate adults. Animals carrying four copies of brBRcore.NS.Z3.hs and heat shocked at 56 hours APF have essentially full length bristles that are unpigmented.
In response to heatshock, ectopic dorsal appendage material is produced in the dorsal anterior region of the eggshell. 3-5 post heatshock none of the eggs produced are abnormal, though only 8% of eggs laid hatch. From 5-24 hours post heatshock 42% of eggs produced are abnormal, and 18% of eggs laid hatch. 24-48 hours post heatshock, about a 1/4 of eggs produced are abnormal, and 90% of eggs laid hatch. Viability is recovered to about 95% in 2 days after heatshock. About 20% of all laid eggs have aberrant micropyles, due to excess chorion formation.
A 37oC heat shock causing expression of brBRcore.NS.Z3.hs during the pupal stage results in lethality. Heat shock induced expression of brBRcore.NS.Z3.hs during larval or prepupal stages has no effect.