Open Close
General Information
Symbol
Dmel\brBRcore.NS.Z3.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0057742
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

br cDNA dm797 is expressed from a heat inducible Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ubiquitous expression of brBRcore.NS.Z3.hs does not cause malrotation of male genitalia.

83% of larvae expressing four copies of brZ3.Scer\UAS induced by heat shock at 5 hours after ecdysis to the second instar fail to initiate the third larval moult but continue to eat and grow, far exceeding the size of a normal second instar larva. These larvae eventually pupariate approximately 93 hours after the heat-shock treatment, forming "L2 prepupae" which are characterised by their uneverted, single club-shaped anterior spiracles and small mouthhooks with a few teeth (characteristic of wild-type second instar larvae). Approximately 97% of the L2 prepupae successfully evert their imaginal discs and head, and most of them develop up to stage P8, showing pale yellow pigmentation in the eyes. In a very few cases, they become pharate adults.

Expression of brBRcore.NS.Z3.hs in second instar larvae induced by heat shock at least 7 hours after ecdysis to the second instar does not prevent the animals forming a pharate third instar, although most of the animals do not ecdyse.

Expression of brBRcore.NS.Z3.hs in early first instar larvae by heat shock at 2-5 hours after hatching results in larvae that ecdyse to the second instar, but with an 18 hour delay at 25[o]C.

Expression of brBRcore.NS.Z3.hs in first instar larvae by heat shock at 4 hours after hatching followed by two heat shocks at approximately 16 hours and 28 hours prevents 96% of the larvae from moulting to the second instar.

Animals carrying four copies of brBRcore.NS.Z3.hs and heat shocked at 48 hours after puparium formation (APF) develop into morphologically normal pharate adults. Animals carrying four copies of brBRcore.NS.Z3.hs and heat shocked at 56 hours APF have essentially full length bristles that are unpigmented.

In response to heatshock, ectopic dorsal appendage material is produced in the dorsal anterior region of the eggshell. 3-5 post heatshock none of the eggs produced are abnormal, though only 8% of eggs laid hatch. From 5-24 hours post heatshock 42% of eggs produced are abnormal, and 18% of eggs laid hatch. 24-48 hours post heatshock, about a 1/4 of eggs produced are abnormal, and 90% of eggs laid hatch. Viability is recovered to about 95% in 2 days after heatshock. About 20% of all laid eggs have aberrant micropyles, due to excess chorion formation.

A 37oC heat shock causing expression of brBRcore.NS.Z3.hs during the pupal stage results in lethality. Heat shock induced expression of brBRcore.NS.Z3.hs during larval or prepupal stages has no effect.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Salivary gland histolysis is not rescued in ftz-f1ex7/ftz-f103649 transheterozygotes when brBRcore.NS.Z3.hs is expressed using heat shock at 10 hours after puparium formation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Expression of the brBRcore.NS.Z3.hs transgene partially rescues the malrotated genitalia phenotype of br2Bc-2 males; this is inversely related to temperature, with 40% of flies showing the phenotype at 22-23oC and only 14% showing the phenotype at 25oC.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

The br Z3 protein isoform encoded by brBRcore.NS.Z3.hs provides full 2Bc+ function.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
brBRcore.NS.Z3.hs
Name Synonyms
Secondary FlyBase IDs
    References (12)