Induction of expression of ftz-f1hs.PM at 25[o]C in animals expressing Cyp18a1GD1176 under the control of Scer\GAL4da.G32 and Scer\GAL80ts.αTub84B rescues the leg malformation phenotype. Only 12% of animals display any leg defects, compared to 87% of control animals.
In ftz-f1ex7/ftz-f103649 mutants, no pupation occurs when ftz-f1hs.PM is induced at 4 or 5hr APF, but 75 or 100% of animals become pupa at 12.3 or 12.6 APF on average when ftz-f1hs.PM is induced at 6 or 7hr APF, respectively.
About 40% of ftz-f1ex7 homozygous embryos hatch when ftz-f1hs.PM is expressed using heat shock at 13-16 hours after egg laying. ftz-f1ex7 lethality is not rescued by heat shock at other times. ftz-f1hs.PM rescues ftz-f1ex7/ftz-f103649 animals that are arrested as prepupae before stage P4; the rescued animals are able to become pupae and a fraction of them develop until at least stage P12. Efficient rescue is seen when the animals are heat shocked 6-10 hours after puparium formation.