FlyBase Allele Report: Hsap\RELA[AD.R52H01]

The R52H01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Tuthill et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

GMR52H01

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage -- third instar larval stage

larval mushroom body output neuron m1

Comment: when combined with P{R40F09-GAL4.DBD} (combination referred to as 'SS02163')

(Eschbach et al., 2021)

third instar larval stage

larval mushroom body output neuron m1

Comment: when combined with P{R40F09-GAL4.DBD} (combination referred to as 'SS02163')

(Eschbach et al., 2020)

pharate adult stage P9

lamina wide-field 1 neuron

Comment: when combined with P{R17C11-GAL4.DBD}

(Astigarraga et al., 2018)

adult stage

mushroom body output neuron 11

Comment: when combined with P{R52B07-GAL4.DBD} (combination referred to as 'MB085C')

(Aso et al., 2014)

lamina wide-field 1 neuron

Comment: when combined with P{R17C11-GAL4.DBD}

(Tuthill et al., 2013)

lamina wide-field 1 neuron | subset

Comment: when combined with P{R17C11-GAL4.DBD} (combination referred to as 'Lawf1b-Gal4')

(Yuan et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R52B07, shi^1.UAS

(Aso et al., 2014)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R52B07, shi^{ts1.IVS.p10.20xUAS}

(Aso et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS, at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R52B07 and Hsap\RELA^AD.R52H01 in adults during training, consolidation and testing significantly reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) significantly reduces aversive 1 min associative visual memory, compared to controls.

(Aso et al., 2014)

Silencing of Lawf1 lamina neurons, using Hsap\RELA^{AD.R52H01.T:Zzzz\Zip+}, Scer\GAL4^{DBD.R17C11.T:Zzzz\Zip-} and Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, abolishes reverse-optomotor inversion at high speeds.

(Tuthill et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement