FlyBase Allele Report: Dmel\AIF[T52]

General Information

Symbol

Dmel\AIF^T52

Species

D. melanogaster

Name

FlyBase ID

FBal0304370

Feature type

allele

Associated gene

Dmel\AIF

Associated Insertion(s)

TI{TI}AIF^T52

Carried in Construct

Also Known As

aif^KO

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

FLPase

(Joza et al., 2008)

gene targeting by homologous recombination

(Joza et al., 2008)

I-SceI

(Joza et al., 2008)

recombination

(Joza et al., 2008)

Progenitor genotype

Associated Insertion(s)

TI{TI}AIF^T52

Cytology

Description

Duplication of the AIF gene. The 5' copy has a frameshifting base pair insertion in both exon 3 and exon 6. The 3' copy of AIF has a frameshifting base pair insertion in exon 3. A single FRT site, a w^+mW.hs marker and a RS(I-CreI) site are present between the duplicated copies.

(Joza et al., 2008)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

RS(I-CreI)

nuclease target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | embryonic stage | maternal effect (with AIF^T2)

(Tarayrah-Ibraheim et al., 2021)

decreased body size | larval stage

(Joza et al., 2008)

decreased cell death | embryonic stage

(Joza et al., 2008)

lethal - all die before end of larval stage

(Joza et al., 2008)

lethal - all die during embryonic stage

(Tarayrah-Ibraheim et al., 2021)

some die during larval stage

(Joza et al., 2008)

Phenotype Manifest In

Bolwig organ

(Joza et al., 2008)

larval ventral nerve cord

(Joza et al., 2008)

primordial germ cell | embryonic stage | maternal effect (with AIF^T2)

(Tarayrah-Ibraheim et al., 2021)

Detailed Description

Statement

Reference

Dp(2;2)AIF^T52 mutant neuroblast clones (carrying both the AIF^T52.5' and AIF^T52.3' mutations) maintain a single neuroblast and consist of many neuronal progeny.

(Kuang et al., 2014)

Homozygotes hatch, but larvae die within several days after egg laying (AEL); approximately half of the larvae are dead by 6 days AEL and by day 8 all the homozygotes have died. The mutant larvae are noticeably smaller than normal within hours after hatching. By day 4 AEL, the mutant larvae are at the 1st and 2nd instar stage (in contrast to wild type, where most larvae have reached the 3rd larval instar by this stage). No third instar larvae are recovered. Growth arrest at L1 of the mutant larvae is exacerbated under crowded culture conditions.

Homozygous embryos show reduced developmental programmed cell death than controls. The pattern of neuronal cells appears unperturbed in the mutant embryos, but extra cells are present in the ventral nerve cord (VNC) and Bolwig's organ. The number of cells in the VNC is normal in abdominal segment A1, but increases in a posterior manner in segments A2-A6 compared to wild type.

Homozygous Dp(2;2)AIF^T52 animals (carrying both the AIF^T52.5' and AIF^T52.3' mutations) hatch, but larvae die within several days after egg laying (AEL); approximately half of the larvae are dead by 6 days AEL and by day 8 all the homozygotes have died. The mutant larvae are noticeably smaller than normal within hours after hatching. By day 4 AEL, the mutant larvae are at the 1st and 2nd instar stage (in contrast to wild type, where most larvae have reached the 3rd larval instar by this stage). No third instar larvae are recovered. Growth arrest at L1 of the mutant larvae is exacerbated under crowded culture conditions.

Homozygous Dp(2;2)AIF^T52 embryos (carrying both the AIF^T52.5' and AIF^T52.3' mutations) show reduced developmental programmed cell death than controls. The pattern of neuronal cells appears unperturbed in the mutant embryos, but extra cells are present in the ventral nerve cord (VNC) and Bolwig's organ. The number of cells in the VNC is normal in abdominal segment A1, but increases in a posterior manner in segments A2-A6 compared to wild type.

(Joza et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

AIF^T52 is a suppressor | partially of abnormal neuroanatomy | somatic clone | larval stage phenotype of fzy⁵⁰³²

(Kuang et al., 2014)

NOT Suppressor of

Statement

Reference

AIF^T52 is a non-suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

AIF^T52 is a suppressor | partially of neuroblast | somatic clone | larval stage phenotype of fzy⁵⁰³²

(Kuang et al., 2014)

NOT Suppressor of

Statement

Reference

AIF^T52 is a non-suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

AIF^T52 partially suppresses the larval neuroblast proliferation defects seen in fzy⁵⁰³² mutant clones: the double mutant clones consistently contain more progeny than a fzy⁵⁰³² single mutant clone, albeit the frequency of neuroblast-containing clones appears similar in both single and double mutant clones.

As in wild type, Dp(2;2)AIF^T52 (which carries both the AIF^T52.5' and AIF^T52.3' mutations) partially suppresses the larval neuroblast proliferation defects seen in fzy⁵⁰³² mutant clones. The frequency of neuroblast-containing clones is similar with or without Dp(2;2)AIF^T52.

(Kuang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

AIF^KO

(Zhang et al., 2016, Yang et al., 2013, Joza et al., 2008)

AIF^T52.3'

AIF^T52.5'

AIF^T52

(Joza et al., 2008)

Dp(2;2)AIF^T52

aif⁵²

(Tarayrah-Ibraheim et al., 2021)

aif^KO

(Kuang et al., 2014)

aif^T52

(Tarayrah-Ibraheim et al., 2021)

Name Synonyms

Secondary FlyBase IDs

FBal0218257
FBal0218256
FBab0045572

References (5)

Report Sections

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