The R81E11 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB543B')
Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB543B')
Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB543B')
Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB543B')
Comment: when combined with P{R65B09-p65.AD} (combination referred to as 'MB543B')
Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R81E11 and Hsap\RELAAD.R65B09 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.
Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R81E11 and Hsap\RELAAD.R65B09 in adults during training, consolidation and testing does not substantially reduce aversive or attractive 2 h associative olfactory memory, or appetitive 1 min associative visual memory, compared to controls; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, and inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.
Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R81E11 and Hsap\RELAAD.R65B09 in adults does not significantly affect sleep, compared to controls, and there is no effect on social or locomotor behaviors.
Split-GAL4 transgene, constructed as described in FBrf0212052.