FlyBase Allele Report: Hsap\RELA[AD.R26E07]

General Information

Symbol

Hsap\RELA^AD.R26E07

Species

H. sapiens

Name

FlyBase ID

FBal0304508

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R26E07-p65.AD}

PBac{R26E07-p65.AD}

Also Known As

R26E07-p65ADZp

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R26E07-p65.AD}

PBac{R26E07-p65.AD}

Cytology

Description

The R26E07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR26E07

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

alpha'/beta' Kenyon cell | intense

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Aso et al., 2014)

alpha/beta core Kenyon cell | faint

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Aso et al., 2014)

alpha/beta posterior Kenyon cell | intense

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Vogt et al., 2016, Aso et al., 2014)

alpha/beta surface Kenyon cell | intense

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Vogt et al., 2016, Aso et al., 2014)

gamma Kenyon cell | intense

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Aso et al., 2014)

alpha'/beta' middle Kenyon cell

Comment: when combined with P{R30F02-GAL4.DBD} (combination referred to as 'MB418B')

(Vogt et al., 2016, Aso et al., 2014)

gamma dorsal Kenyon cell

Comment: when combined with P{R39A11-GAL4.DBD} (combination referred to as 'MB419B')

(Vogt et al., 2016, Aso et al., 2014)

alpha'/beta' anterior-posterior Kenyon cell | intense

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Vogt et al., 2016)

alpha'/beta' middle Kenyon cell | intense

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Vogt et al., 2016)

alpha/beta core Kenyon cell

Comment: when combined with P{R29G11-GAL4.DBD} (combination referred to as 'MB417B')

(Vogt et al., 2016)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R26E07 significantly impairs visual (aversive color and brightness discrimination conditioning) but not olfactory (aversive conditioning) memory performance, compared to controls.

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4^DBD.R29G11 and Hsap\RELA^AD.R26E07 or Scer\GAL4^DBD.R30F02 and Hsap\RELA^AD.R26E07 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

(Vogt et al., 2016)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R29G11 and Hsap\RELA^AD.R26E07 does not significantly affect the amount of sleep in adult males or females, compared to controls, and there is no effect on locomotor activity of awake adult males; activation of neurons targeted by Scer\GAL4^DBD.R39A11 and Hsap\RELA^AD.R26E07 significantly increases the amount of sleep in adult females, but not males, compared to controls, with no effect on locomotor activity of awake adult males; activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R30F02 and Hsap\RELA^AD.R26E07 significantly decreases the amount of sleep in adult males or females, compared to controls, and significantly decreases locomotor activity of awake adult males.

(Sitaraman et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R16H11 and Hsap\RELA^AD.R26E07 in adults does not affect social or locomotor behaviors, compared to controls.

(Aso et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions