FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\RELAAD.R64F07
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General Information
Symbol
Hsap\RELAAD.R64F07
Species
H. sapiens
Name
FlyBase ID
FBal0304524
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R64F07-p65.AD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Aso et al., 2014)
Progenitor genotype
Carried in construct
P{R64F07-p65.AD}
Cytology
Description

The R64F07 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
GMR64F07
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      mushroom body output neuron 22

      Comment: when combined with P{R57C10-GAL4.DBD}

      when combined with P{R57C10-GAL4.DBD} (combination referred to as 'MB242A')

      when combined with P{R64F07-GAL4.DBD} (combination referred to as 'MB622B')

      (Ichinose et al., 2021, Tison et al., 2020, Aso et al., 2014, Aso et al., 2014)
      mushroom body output neuron 22 | intense

      Comment: when combined with P{R64F07-GAL4.DBD} (combination referred to as 'MB622B')

      (Aso et al., 2014)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adult males does not significantly affect the amount of sleep, compared to controls; activation significantly increases locomotor activity of awake adult males, compared to controls.

      (Sitaraman et al., 2015)

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

      Inhibition (using shits1.IVS.p10.20xUAS, but not shi1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adults during training, consolidation and testing substantially reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) does not affect attractive 2 h associative olfactory memory, compared to controls. Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) does not significantly affect aversive or appetitive 1 min associative visual memory, compared to controls; this inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, and inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

      Thermogenetic activation (using TrpA1UAS.(B).cKa, but not TrpA120xUAS.JFRC124, at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adults significantly increases sleep, compared to controls, with no effect on social or locomotor behaviors.

      Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R64F07 and Hsap\RELAAD.R64F07 in adults significantly increases sleep, compared to controls.

      (Aso et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (4)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Aso et al., 2014)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R64F07.T:Zzzz\Zip+
      Hsap\RELAAD.R64F07
      Name Synonyms
      Secondary FlyBase IDs
        References (13)