The R57C10 enhancer fragment and promoter is followed the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.
Comment: when combined with P{R64F07-p65.AD}
when combined with P{R64F07-p65.AD} (combination referred to as 'MB242A')
Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adult males does not significantly affect the amount of sleep, compared to controls; activation significantly increases locomotor activity of awake adult males, compared to controls.
Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.
Inhibition (using shits1.IVS.p10.20xUAS, but not shi1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adults during training, consolidation and testing substantially reduces aversive 2 h associative olfactory memory, compared to controls; inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) does not affect attractive 2 h associative olfactory memory, compared to controls. Inhibition (using shits1.IVS.p10.20xUAS at 31-33[o]C) does not significantly affect aversive or appetitive 1 min associative visual memory, compared to controls; this inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, and inhibition only during training and testing does not significantly reduce 24 h appetitive, or 30 min aversive, associative olfactory memory towards ethanol, compared to controls.
Thermogenetic activation (using TrpA1UAS.(B).cKa, but not TrpA120xUAS.JFRC124, at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R57C10 and Hsap\RELAAD.R64F07 in adults significantly increases sleep, compared to controls, with no effect on social or locomotor behaviors.
Split-GAL4 transgene, constructed as described in FBrf0212052.