FB2026_02 , released June 18, 2026
Allele: Dmel\mir-bftsponge.V2.UAS.mCherry
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General Information
Symbol
Dmel\mir-bftsponge.V2.UAS.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0305003
Feature type
allele
Associated gene
Dmel\mir-bft
Associated Insertion(s)
Carried in Construct
P{UAS-mCherry.mir-bft.sponge.V2}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Fulga et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-mCherry.mir-bft.sponge.V2}
Cytology
Description

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

(Fulga et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
mir-bft
Tagged with
mCherry
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The lack of a Scer\GAL4tub.PU-mediated viability phenotype is a false negative (compared to independent viability data from miRNA deletion mutants).

      (Fulga et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      bftsponge.V2.Scer\UAS.T:Disc\RFP-mCherry
      bftsponge.V2.UAS.mCherry
      mir-bftsponge.V2.UAS.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (3)